J Periodontal Implant Sci.  2013 Aug;43(4):183-190. 10.5051/jpis.2013.43.4.183.

Quantitative analysis of periodontal pathogens present in the saliva of geriatric subjects

Affiliations
  • 1Department of Oral & Maxillofacial Surgery, Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea.
  • 2Department of Dental Education, Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea. swlee@chonnam.ac.kr
  • 3Department of Periodontology, Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea.
  • 4Department of Oral Pathology, Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea.
  • 5Department of Orthodontics, Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea.
  • 6Department of Periodontics, Marquette University School of Dentistry, Milwaukee, WI, USA.

Abstract

PURPOSE
At present, information regarding periodontal disease in geriatric patients is scarce. The purpose of this study was to quantify the periodontal pathogens present in the saliva of Korean geriatric patients and assess the relationship between the bacterial levels and the periodontal condition.
METHODS
Six putative periodontal pathogens were quantified by using a real-time polymerase chain reaction assay in geriatric patient groups (>60 years) with mild chronic periodontitis (MCP), moderate chronic periodontitis (MoCP), and severe chronic periodontitis (SCP). The copy numbers of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Prevotella intermedia were measured.
RESULTS
It was found that the bacterial copy numbers increased as the severity of the disease increased from MCP to SCP, except for P. intermedia. For P. intermedia, it was found that samples in the MCP group yielded the largest amount. It was also found that the quantities of P. gingivalis, T. forsythia, and T. denticola, the so-called "red complex" bacteria, were lower than those of F. nucleatum, A. actinomycetemcomitans, and P. intermedia in all of the samples.
CONCLUSIONS
Collectively, the results of this study suggest that the levels of P. gingivalis, T. forsythia, F. nucleatum, and T. denticola present in saliva are associated with the severity of periodontal disease in geriatric patients.

Keyword

Chronic periodontitis; Disease progression; Geriatrics; Microbiology; Real-time polymerase chain reaction; Saliva

MeSH Terms

Bacteria
Chronic Periodontitis
Disease Progression
Forsythia
Fusobacterium nucleatum
Geriatrics
Humans
Periodontal Diseases
Porphyromonas gingivalis
Prevotella intermedia
Real-Time Polymerase Chain Reaction
Saliva
Treponema denticola

Figure

  • Figure 1 Box plot to describe multiple comparisons between the groups for Log10 copy cell numbers/mL of (A) Porphyromonas gingivalis, (B) Tannerella forsythia, (C) Treponema denticola, (D) Fusobacterium nucleatum, (E) Aggregatibacter actinomycetemcomitans, and (F) Prevotella intermedia. Mild chronic periodontitis (MCP, n=29), moderate chronic periodontitis (MoCP, n=29), and severe chronic periodontitis (SCP, n=30). The box represents the first and third quartiles. The line within the box represents the median value. *Significant difference (P≤0.05). **Significant difference (P≤0.01). ***Significant difference (P≤0.001). CFU: colony forming unit.


Cited by  2 articles

Humoral immune responses to periodontal pathogens in the elderly
Uttom Shet, Hee-Kyun Oh, Hyun-Ju Chung, Young-Joon Kim, Ok-Su Kim, Hoi-Jeong Lim, Min-Ho Shin, Seok-Woo Lee
J Periodontal Implant Sci. 2015;45(5):178-183.    doi: 10.5051/jpis.2015.45.5.178.

Real-time PCR quantification of 9 periodontal pathogens in saliva samples from periodontally healthy Korean young adults
Heeyoung Choi, Eunhye Kim, Jihoon Kang, Hyun-Joo Kim, Ju-Youn Lee, Jeomil Choi, Ji-Young Joo
J Periodontal Implant Sci. 2018;48(4):261-271.    doi: 10.5051/jpis.2018.48.4.261.


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