J Korean Soc Transplant.
2006 Jun;20(1):49-54.
The Effect of alpha-Melanocyte Stimulating Hormone on Renal Ischemia Reperfusion Injury
- Affiliations
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- 1Department of Surgery, Ewha Womans University College of Medicine, Korea.
- 2Department of Pathology, Ewha Womans University College of Medicine, Korea.
- 3Department of Anatomy, Ewha Womans University College of Medicine, Korea.
- 4Department of Clinical Pathology, Ewha Womans University College of Medicine, Korea.
- 5Department of Surgery, Yonsei University College of Medicine, Korea.
- 6Kidney Research Center, Ewha Womans University College of Medicine, Seoul, Korea. kuyong@ewha.ac.kr
Abstract
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PURPOSE: In ischemia-reperfusion induced renal injuries, cytokines, chemoattractant chemokines, adhesion molecules and nitric oxide play an important role. alpha-Melanocyte stimulating hormone (alpha-MSH) is a potent anti-inflammatory cytokine so the therapeutic effect of alpha-MSH on an ischemia-reperfusion induced acute renal failure is to be evaluated.
METHODS
40 male Spraque-Dawley rats were prepared for the experiment, they were classified into three classes (Sham, ischemic control and alpha-MSH injection). Both renal pedicles were clamped for 45 minutes. alpha-MSH (50 microgram) was injected intravenously three times, immediately before ischemia and reperfusion and 18 hour after reperfusion. Serum creatinine and histologic changes were analyzed between groups (Sham (n=6), ischemic control group (n=15), and alpha-MSH group (n=19)).
RESULTS
Serum creatinine level decreased significantly at 24 hours after reperfusion in alpha-MSH treated animals (SCr24 0.78+/-0.23 mg/dL, 4.21+/-1.14 mg/dL, 3.01+/-1.19 mg/dL, repectively (P=0.008)), especially serum creatinine level at 48 hours after reperfusion much more dicreased in alpha-MSH group (SCr48 0.67+/-0.16 mg/dL, 4.21+/-2.03 mg/dL, 1.15+/-1.11 mg/dL, repectively (P=0.004)). Tubular neccrosis and neutrophil infiltration decreased signigicantly in alpha-MSH treated group (P=0.001). Mortality was noted 33.3% only at ischemic conrol group.
CONCLUSION
we demonstrate the fact that alpha-MSH has protective role on ischemic renal injury and improves survival rates.
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