J Korean Acad Periodontol.  2007 Dec;37(4):849-857. 10.5051/jkape.2007.37.4.849.

The Expression of RANK and RANKL in Gingival Tissue of Human Chronic Periodontitis

Affiliations
  • 1Department of Periodontology, School of Dentistry, Kyungpook National University, Korea. leejm@knu.ac.kr

Abstract

PURPOSE
The purposes of this study were to compare and quantify the expressions of RANK and RANKL in the gingival tissues of non-periodontitis patient and patients with chronic periodontitis, in order to understand the contribution of these proteins to periodontal destruction. MATERIAL AND METHODS: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was divided into two groups. Group 1 (n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from non-periodontitis patients. Group 2 (n=8) is inflammed gingiva from patients with chronic periodontitis. Tissue samples were prepared and analyzed by Western blotting. The quantification of RANK and RANKL were performed using a densitometer and statistically analyzed by Student's t-Test.
RESULTS
The expression of RANK were similar in group 1 and 2. The difference between group 1 and 2 was not statistically significant. And the mean amount of RANKL was more increased in group 2 than group 1. The difference between group 1 and group 2 was statistically significant.
CONCLUSION
The expression level of RANK didn't show any significant difference between healthy tissue from non-periodontitis patients and inflamed tissue from chronic periodontitis, but the expression level of RANKL in inflammed tissue from chronic periodontitis showed significantly increased tendency compared to healthy gingiva from non-periodontitis patients. Threrefore, characteristics of RANK and RANKL in progress of chronic periodontitis would be basis of further studies in diagnostic method and treatment index of the disease.

Keyword

Chronic periodontitis; RANK; RANKL; gingival tissue

MeSH Terms

Blotting, Western
Bone Resorption
Chronic Periodontitis*
Gingiva
Hemorrhage
Humans*
Periodontal Pocket
Tooth Extraction
Full Text Links
  • JKAPE
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr