Korean J Vet Res.  2011 Mar;51(1):29-35.

Isolation and Antimicrobial Susceptibility of Microorganisms from Milk Samples of Jindo Dogs (Canis familiaris var. jindo)

• Lee JD
• Lee YK
• O SI
• Jung JY
• Son CH
• Shin SS
• Oh KS
• Hur TY
• Suh GH

Abstract

Lack of hygiene and puerperal mastitis are common causes of bacterial diseases in nursing neonates. The aim of this study was to isolate microorganisms from milk samples of healthy female Jindo dogs with suckling puppies and to investigate antimicrobial susceptibility against the isolated bacteria. Milk samples were collected from 120 udders of 12 lactating Jindo dogs that were 2~4 years old without any clinical diseases including mastitis. Bacteria were isolated from 64 milk samples (53.3%), either singly (76.6%) or in combination (23.4%). Staphylococcus (S.) spp. was the most common microorganisms (74.7%) isolated from canine milk, followed by Haemophillus spp. (10.9%), Streptococcus spp. (9.6%), Gardnerella spp. (2.4%) and Moraxella spp. (2.4%). The most frequently isolated organism was S. warneri (31.3%). Antimicrobial susceptibility of these bacteria was tested with 17 antimicrobial agents by Kirbyand Bauer standardized disc diffusion method. Results indicated that bacteria isolated from healthy canine milk were mostly susceptible to amoxicillin-clavulanic acid, cephalothin and ceftiofur, but were resistant to erythromycin, neomycin and tetracycline.

Keyword

antimicrobial suseptibility; canine mastitis; milk

MeSH Terms

Amoxicillin-Potassium Clavulanate Combination
Animals
Anti-Infective Agents
Bacteria
Cephalosporins
Cephalothin
Diffusion
Dogs
Erythromycin
Female
Gardnerella
Humans
Hygiene
Infant, Newborn
Mammary Glands, Animal
Mastitis
Milk
Moraxella
Neomycin
Staphylococcus
Streptococcus
Tetracycline
Amoxicillin-Potassium Clavulanate Combination
Anti-Infective Agents
Cephalosporins
Cephalothin
Erythromycin
Neomycin
Tetracycline