Korean J Nucl Med.  2000 Feb;34(1):74-81.

Development of a Noble Dosimetry Using Metaphase Analysis and Micronuclei Assay of Bone Marrow Cells in Mice

• Min JJ
• Bom HS
• Kim YH
• Yoon HJ
• Kim JY

Abstract

PURPOSE: The purpose of this study was to develop in vivo dosimetries using both chromosomal aberrations and micronuclei in mice to assess biological effects of radiations.
MATERIALS AND METHODS
Five each mice were irradiated with 0, 1, 2, 3, 4, 5, 10 Gy of Cs-137 gamma-rays. We scored numbers of chromosomal aberrations in metaphase spreads and numbers of micronuclei in bone marrow smears under light microscope, and obtained the dose-response relationships. We also examined the relationship between the two dose-response curves.
RESULTS
The frequency of both chromosomal aberrations and micronuclei increased with dose, in a linear-quadratic manner. The delta, beta, and alpha coefficients were 0.0176, 0.0324, and 0.0567 for metaphase analysis (r=1.0, p<0.001) and 0.0019, 0.0073, and 0.0506 for micronuclei assay (r=1.0, p<0.001). The frequency of chromosomal aberrations and micronuclei in diffirent radiation doses was significantly correlated (r=0.99, p<0.01).
CONCLUSION
In vivo dosimetry using either metaphase analysis or micronucleus assay was feasible in mice. These methods could be useful to evaluate biological effects of radiation.

Keyword

Bone marrow; Chromosomal aberration; Micronucleus; Metaphase analysis; Micronuclei assay; Biological dosimetry

MeSH Terms

Animals
Bone Marrow Cells*
Bone Marrow*
Chromosome Aberrations
Metaphase*
Mice*
Micronucleus Tests