Korean J Nephrol.
2002 Nov;21(6):956-965.
Effect of Transforming Growth Factor-beta1 (TGF-beta1) on the Expression of Vascular cell Adhesion Molecule-1 (VCAM-1) in Cultured Human Peritoneal Mesothelial Cells (HPMCs)
- Affiliations
-
- 1Department of Internal Medicine, Kangwon National University, Korea.
- 2Departments of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea. wsyang@amc.seoul.kr
- 3Departments of Surgery, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
Abstract
-
BACKGROUND: In early phase of peritonitis, mononuclear cells as well as polymorphonuclear leukocytes migrate rapidly into peritoneal cavity. For the migration of mononuclear cells, the expression of VCAM-1 on peritoneal mesothelial cells is important. In this study, we investigated the effect of TGF-beta1 on tumor necrosis factor-alpha (TNF-alpha) or interleukin-1beta(IL-1beta) induced VCAM-1 expression in the cultured HPMCs.
METHODS
HPMCs were cultured in the presence of TNF-alpha, IL-1beta and/or TGF-beta1. VCAM-1 mRNA level was measured by Northern blot. VCAM-1 in total cell lysate and VCAM-1 expressed on cell surface were measured by Western blot and cellular ELISA, respectively.
RESULTS
Incubation of the cultured HPMCs with TNF-alpha (10 ng/mL) or IL-1beta (1 ng/mL) caused an increased level of VCAM-1 mRNA, VCAM-1 protein in total cell lysate, and VCAM-1 expressed on cell surface. This stimulatory effects of TNF-alpha or IL- 1beta were inhibited by TGF-beta1 (0.1, 1, 10 ng/mL), dose-dependently. The level of VCAM-1 mRNA, VCAM-1 protein in total cell lysate, and VCAM-1 expressed on cell surface in the unstimulated cells were also inhibited by TGF-beta1 (10 ng/mL). The rate of VCAM-1 mRNA degradation after an application of actinomycin D was not affected by TGF-beta1.
CONCLUSION
TGF-beta1 inhibited inflammatory cytokine induced VCAM-1 production and expression in the cultured HPMCs. Treatment of the cells with TGF-beta1 seems to suppress TNF-alpha or IL-1beta induced VCAM-1 mRNA transcription rather than decrease stabilization of VCAM-1 mRNA.
Keyword
MeSH Terms
-
Blotting, Northern
Blotting, Western
Dactinomycin
Enzyme-Linked Immunosorbent Assay
Humans*
Neutrophils
Peritoneal Cavity
Peritonitis
RNA Stability
RNA, Messenger
Transforming Growth Factor beta1
Tumor Necrosis Factor-alpha
Vascular Cell Adhesion Molecule-1*
Dactinomycin
RNA, Messenger
Transforming Growth Factor beta1
Tumor Necrosis Factor-alpha
Vascular Cell Adhesion Molecule-1
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The Effect of Dexamethasone and Transforming Growth Factor-beta1 on the Cytokine Induced Expression of VCAM-1 in Glomerular Endothelial Cells
- Transforming Growth Factor-beta1(TGF-beta1) Synthesis of Human Peritoneal Mesothelial Cell
- Inhibition of Cell Growth and Suppression of c-myc Gene Expression by Transforming Growth Factor-beta1 in Cervical Carcinoma Cell Lines
- The Transforming Growth Factor-beta1 Expression in Normal Laryngeal Mucosa, Laryngeal Dysplasia and Laryngeal Carcinoma
- Expression of Transforming Growth Factor -beta1, -beta2 in Human Endometrium of The Uterine Adenocarcinoma
