J Korean Acad Conserv Dent.  2008 Mar;33(2):141-147. 10.5395/JKACD.2008.33.2.141.

Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid

  • 1Department of Conservative Dentistry, School of Dentistry, Seoul National University, Korea. inboglee@snu.ac.kr


This study was aimed to develop an instrument for real-time measurement of fluid conductance and to investigate the hydrodynamics of dentinal fluid. The instrument consisted of three parts; (1) a glass capillary and a photo sensor for detection of fluid movement, (2) a servo-motor, a lead screw and a ball nut for tracking of fluid movement, (3) a rotary encoder and software for data processing. To observe the blocking effect of dentinal fluid movement, oxalate gel and self-etch adhesive agent were used. BisBlock (Bisco) and Clearfil SE Bond (Kuraray) were applied to the occlusal dentin surface of extracted human teeth. Using this new device, the fluid movement was measured and compared between before and after each agent was applied. The instrument was able to measure dentinal fluid movement with a high resolution (0.196 nL) and the flow occurred with a rate of 0.84 to 15.2 nL/s before treatment. After BisBlock or Clearfil SE Bond was used, the fluid movement was decreased by 39.8 to 89.6%.


Dentinal tubule; Water conductance; Dentin hypersensitivity; Oxalate; Self etching adhesive; Hydrodynamics

MeSH Terms

Dentin Sensitivity
Dentinal Fluid
Oxalic Acid
Resin Cements
Track and Field
Oxalic Acid
Resin Cements


  • Figure 1-a Schematic diagram of the nano-flow measurement device.

  • Figure 1-b Circuit diagram of photo sensor and servo amplifier.

  • Figure 2 Representative curves of dentinal fluid flow before and after application of BisBlock (a) or Clearfil SE bond (b).

Cited by  2 articles

Real-time measurement of dentinal tubular fluid flow during and after amalgam and composite restorations
Sun-Young Kim, Byeong-Hoon Cho, Seung-Ho Baek, Bum-Sun Lim, In-Bog Lee
J Korean Acad Conserv Dent. 2009;34(6):467-476.    doi: 10.5395/JKACD.2009.34.6.467.

Real-time measurement of dentinal fluid flow during desensitizing agent application
Sun-Young Kim, Eun-Joo Kim, In-Bog Lee
J Korean Acad Conserv Dent. 2010;35(5):313-320.    doi: 10.5395/JKACD.2010.35.5.313.


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