J Korean Acad Conserv Dent.  2009 Nov;34(6):500-507. 10.5395/JKACD.2009.34.6.500.

Comparison of Antibacterial effect of Listerine(R) with Various root canal irrigants

• Kim YH
• Kang MK
• Choi EK
• Yang SY
• Yang I
• Kang IC
• Hwang YC
• Hwang IN
• Oh WM

• KMID: 1986613
• DOI: http://doi.org/10.5395/JKACD.2009.34.6.500

Abstract

The purpose of this study is to compare the antibacterial effect of Listerine(R) on two microorganisms (P. gingivalis and E. faecalis) with various root canal irrigants (NaOCl, CHX, EDTA) and to identify possibility of using Listerine(R) as a root canal irrigant. Porphyromonas gingivalis ATCC 3327 and Enterococcus faecalis ATCC 29212 were used in this experiment. For the test irrigants, 0.5%, 1%, 2.5%, 5.25% NaOCl, 0.1%, 0.2%, 1%, 2% CHX, 0.5M EDTA (18.6% EDTA) and Listerine(R) were prepared. Distiled water was used as control. Two methods-1) Comparison of turbidity in broth and 2) Agar diffusion test-were used to determine the extent of antibacterial effect of Listerine(R) and to compare it with that of NaOCl, CHX, and EDTA. All solutions tested were effective against two bacterial strains compared with control (p<0.001). Any concentration of NaOCl, CHX, and EDTA showed similarly high effectiveness against all bacterial strains. In all experiment, Listerine(R) showed significantly low antibacterial effect compared with the other root canal irrigants (p<0.05). In conclusion, the results reflect remarkably low antibacterial effect of Listerine(R) as compared with root canal irrigants in general so it is not suitable for the root canal irrigant.

Keyword

Root canal irrigant; Antibacterial effect; Essential oil; Listerine(R)

MeSH Terms

Agar
Dental Pulp Cavity
Diffusion
Edetic Acid
Enterococcus faecalis
Porphyromonas gingivalis
Root Canal Irrigants
Water
Agar
Edetic Acid
Root Canal Irrigants
Water

Figure

• 

  Figure 1 Comparison of bacterial turbidity in broth at 630nm wave length after 48hours culture. A. Turbidity of E. faecalis at 630 nm B. Turbidity of P. gingivalis at 630 nm -C0.1: 0.1% CHX, C0.2: 0.2% CHX, C1: 1% CHX, C2: 2% CHX, N0.5: 0.5% NaOCl, N1: 1% NaOCl, N2.5: 2.5% NaOCl, N5.25: 5.25% NaOCl, DW: Distilled water(Control) **: Statistical significance (p<0.001) *: Statistical significance (p<0.05)

• 

  Figure 2 Comparison of agar diffusion test after 48hours culture (Averages of zones of inhibition)(mm). A. Diameter of inhibition zone of E. faecalis B. Diameter of inhibition zone of P. gingivalis -C0.1: 0.1% CHX, C0.2: 0.2% CHX, C1: 1% CHX, C2: 2% CHX, N0.5: 0.5% NaOCl, N1: 1% NaOCl, N2.5: 2.5% NaOCl, N5.25: 5.25% NaOCl, DW: Distilled water(Control) **: Statistical significance (p<0.001) *: Statistical significance (p<0.05)

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