J Bone Metab.
2012 Nov;19(2):121-127. 10.11005/jbm.2012.19.2.121.
Effect of Cornus Officinalis on Receptor Activator of Nuclear Factor-kappaB Ligand (RANKL)-induced Osteoclast Differentiation
- Affiliations
-
- 1Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Korea. jjkim@wku.ac.kr
- 2Department of Oriental Pharmacy, Wonkwang University, Iksan, Korea.
- 3Wonkwang Oriental Medicines Research Institute, Wonkwang University, Iksan, Korea.
- 4Institute for Skeletal Disease, School of Medicine, Wonkwang University, Iksan, Korea.
- 5Institute for Environmental Science, School of Medicine, Wonkwang University, Iksan, Korea.
- KMID: 1975985
- DOI: http://doi.org/10.11005/jbm.2012.19.2.121
Abstract
OBJECTIVES
Osteoporosis is a disease of bones that is thought to result from an imbalance between bone resorption and bone formation. Although osteoporosis itself has no symptoms, osteoporosis caused by osteoclasts leads to an increased risk of fracture. Here we examined the effects of cornus officinalis on receptor activator of nuclear factor-kappaB ligand (RANKL)-mediated osteoclast differentiation.
METHODS
We evaluated the effects of cornus officinalis on RANKL-induced osteoclast differentiation from bone marrow-derived macrophages (BMMs) and performed a cytotoxicity assay, reverse transcriptase-polymerase chain reaction (RT-PCR), and Western blot analysis.
RESULTS
Cornus officinalis significantly inhibits RANKL-mediated osteoclast differentiation in a dose-dependent manner, but without cytotoxicity against BMMs. The mRNA expression of tartrate-resistant acid phosphatase (TRAP), osteoclast-associated receptor (OSCAR), c-Fos, and nuclear factor of activated T cells cytoplasmic 1 (NFATc1) in BMMs treated with RANKL was considerably inhibited by cornus officinalis treatment. Also, cornus officinalis inhibits the protein expression of c-Fos and NFATc1. Cornus officinalis greatly inhibits RANKL-induced phosphorylation of p38 and c-JUN N-terminal kinase (JNK). Also, cornus officinalis significantly suppresses RANKL-induced degradation of I-kappaB.
CONCLUSIONS
Taken together, our results suggest that cornus officinalis may be a useful the treatment of osteoporosis.
Keyword
MeSH Terms
-
Acid Phosphatase
Blotting, Western
Bone Resorption
Cornus
Cytoplasm
Isoenzymes
JNK Mitogen-Activated Protein Kinases
Macrophages
Osteoclasts
Osteogenesis
Osteoporosis
Phosphorylation
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
RNA, Messenger
T-Lymphocytes
Acid Phosphatase
Isoenzymes
JNK Mitogen-Activated Protein Kinases
RANK Ligand
RNA, Messenger
Receptor Activator of Nuclear Factor-kappa B
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