J Bacteriol Virol.  2004 Sep;34(3):239-246.

Characterization of E. coli-induced Genes in Bombyx mori Fatbody using Expressed Sequence Tags Analysis

Affiliations
  • 1Department of Microbiology and Institute for Viral Diseases, Division of Brain Korea 21 Program for Biomedical Science, College of Medicine, Korea University, Seoul 136-705, Korea.
  • 2Laboratory of Genetics, Department of Life Science, College of Natural Science, Ewha Womans University, Seoul 120-750, Korea.
  • 3Department of Science Education, College of Education, Ewha Womans University, Seoul 120-750, Korea. sjkang@ewha.ac.kr

Abstract

To accelerate the molecular analysis of specifically induced antibacterial peptide against pathogen (E. coli), cDNA library prepared from the larvae fatbody of Bombyx mori was examined by the expressed sequence tag (EST) analysis. In a total of 722 clones, 653 clones were unique genes. Of 653 unique genes, 43.2% (282/653 ESTs) was identified as characterized genes, 38.1% (249/653 ESTs) as uncharacterized genes, and 18.7% (122/653 ESTs) as novel ESTs. According to the functional categorization of the characterized genes, 36.2% (102/282 ESTs) was antibacterial proteins. The highest expressed peptides, 78.4% of all the expressed antibacterial proteins (80/102 ESTs), belonged to the cecropin family. The antibacterial effect of selected clones representing novel ESTs based on a phylogenetic analysis was examined against various bacterial strains. None of the clones showed significant inhibitory effect to the bacteria tested. These results suggested that most of the novel molecules induced by E. coli may not act as immune-induced antibacterial peptides in the fatbody.

Keyword

Antibacterial protein; Bombyx mori; Expressed sequence tag (EST)

MeSH Terms

Bacteria
Bombyx*
Clone Cells
Expressed Sequence Tags*
Gene Library
Humans
Larva
Peptides
Peptides
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