Korean J Leg Med.  2013 Nov;37(4):220-223. 10.7580/kjlm.2013.37.4.220.

DNA Profiling via Short Tandem Repeat Analysis by Using Serum Samples

• Lee JH
• Lee HY
• Cho S
• Cho JY
• Jang IJ
• Lee SD

• KMID: 1928020
• DOI: http://doi.org/10.7580/kjlm.2013.37.4.220

Abstract

Serum is free of cellular components. Because DNA is located in the nuclei or mitochondria of cells, serum could be assumed DNA free. Few previously published case reports to date have used serum for DNA typing. Here, we report on human genotyping via short tandem repeat (STR) analysis using serum as a sample, and discuss problems involved in the process.

Keyword

Serum; DNA Extraction; STR

MeSH Terms

DNA Fingerprinting*
DNA*
Humans
Microsatellite Repeats*
Mitochondria
DNA

Figure

• 

  Fig. 1. The former electropherogram (a, c, e) obtained from blood, and the latter (b, d, f) obtained from serum. (b) indicates allele drop-out of the serum's 11 allele at locus TPOX and 10 allele at locus D5S818. (d) indicates allele drop-in of the serum's 28 allele at locus D2S1338 and allele drop-out of the serum's 15 allele at locus D18S51. (f) indicates drop-in of the serum's 26 allele and drop-out of the 21, 23 allele at locus D22S1045. Also indicates unamplified at locus D19S433.

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