Infect Chemother.  2015 Mar;47(1):33-40. 10.3947/ic.2015.47.1.33.

Characteristics of Metallo-beta-Lactamase-Producing Pseudomonas aeruginosa in Korea

• Hong JS
• Kim JO
• Lee H
• Bae IK
• Jeong SH
• Lee K

• KMID: 1909521
• DOI: http://doi.org/10.3947/ic.2015.47.1.33

Abstract

BACKGROUND
The aim of this study was to investigate the molecular epidemiological characteristics of metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa clinical isolates in Korea.
MATERIALS AND METHODS
Three hundred and twenty nine P. aeruginosa clinical isolates were collected from 23 general hospitals in Korea from March to June 2014. Species were identified by matrix-assited laser desorption/ionization-time of flight and 16S rRNA sequencing. Antimicrobial susceptibility was determined by disk diffusion methods. Further, minimum inhibitory concentrations of carbapenems were determined by Etest. Polymerase chain reaction and sequencing were performed to identify genes encoding MBLs. Multi-locus sequence typing and pulsed-field gel electrophoresis were performed to determine epidemiological characteristics of MBL-producing P. aeruginosa isolates.
RESULTS
Of the 329 isolates, 229 (69.6%) were susceptible to the carbapenems tested, including imipenem and meropenem; while 100 (30.4%) were non-susceptible to more than one of the carbapenems. Genes encoding imipenemase-6 (IMP-6) and Verona imipenemase-2 (VIM-2) MBLs were identified in 21 (6.4%) isolates (n = 17 and 4, respectively). All MBL-producing isolates showed multi-drug resistant phenotype, and a majority (n = 19) of the isolates were identified as sequence type 235 (ST235). The remaining isolates (n = 2) were identified as ST309 and ST463.
CONCLUSION
P. aeruginosa ST235 might play an important role in dissemination of MBL genes in Korea.

Keyword

Pseudomonas aeruginosa; metallo-beta-lactamase; VIM-2 metallo-beta-lactamase; International clone; Multi-locus sequence typing

MeSH Terms

Carbapenems
Diffusion
Electrophoresis, Gel, Pulsed-Field
Hospitals, General
Imipenem
Korea
Microbial Sensitivity Tests
Phenotype
Polymerase Chain Reaction
Pseudomonas aeruginosa*
Carbapenems
Imipenem

Figure

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  Figure 1 Map showing the locations of the participating hospitals in this study. MBL, metallo-β-lactamase; IMP, imipenemase; VIM, Verona imipenemase.

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  Figure 2 XbaI-macrorestriction patterns of metallo-β-lactamase-producing Pseudomonas aeruginosa clinical isolates.

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