Korean J Pediatr Gastroenterol Nutr.
2001 Apr;4(1):34-40.
Comparison among Diagnostic Methods of Rotaviral Gastroenteritis in Children
- Affiliations
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- 1Department of Pediatrics, College of Medicine, Korea University, Seoul, Korea. leejmd@chollian.net
- 2Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.
- 3Department of Pediatrics, Seoul Adventist Hospital, Seoul, Korea.
Abstract
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PURPOSE: The purpose of this study was to evaluate the clinical efficacy of reverse transcription-
polymerase chain reaction (RT-PCR) in detecting rotaviral gastroenteritis in children
comparing with that of commercial immunoassays.
METHODS
Stools from 79 children admitted Korea University Hospital due to diarrhea were collected from December 1999 to February 2000. Immunoassays were done using commercial rotavirus Latex kit and Rotatec (ELISA) kit. RT-PCR was performed to amplify group A rotavirus, most commonly pathogenic to human, using VP4- and VP7-specific primers. The detection rates of immunoassays and RT-PCR were compared.
RESULTS
ELISA assay was superior to LA assay and moderately concordant with RT-PCR in detecting rotaviral gastroenteritis.
CONCLUSION
Although RT-PCR is known very sensitive, it does not have significant advantage over immunoassay in detecting rotaviral gastroenteritis.
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