Korean J Vet Res.  2013 Sep;53(3):163-167.

Evaluation of systemic and mucosal immune responses in mice administered with recombinant Salmonella Typhimurium expressing IutA protein

Affiliations
  • 1College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea. johnhlee@chonbuk.ac.kr

Abstract

Avian pathogenic Escherichia coli (APEC) are known to cause extraintestinal disease in poultry, leading to substantial losses in the industry. IutA, iron-regulated aerobactin receptor is firmly associated with APEC. To assess the potential of IutA to induce protective immune responses, attenuated Salmonella Typhimurium strain expressing IutA was constructed and administered orally to BALB/c mice. The IutA-specific immune responses were measured with sera, vaginal and fecal samples by an enzyme-linked immunosorbent assay. We found that the Salmonella-IutA vaccine induced significantly higher immune responses as compared to the control inoculated with the attenuated S. Typhimurium containing the plasmid only. The IutA-specific immune responses were increased by second immunization at third week after initial immunization, whereas triple immunization induced lower immune responses than those induced by the double immunization. The Salmonella-IutA vaccine induced a nature of immunity biased to the Th1-type, as judged by the ratio of IutA-specific IgG isotypes (IgG2a/IgG1). Overall, these results suggest that the Salmonella-IutA vaccine appear to be suitable candidate for a vaccine against APEC.

Keyword

avian pathogenic Escherichia coli; boost immunization; IutA; oral vaccination

MeSH Terms

Animals
Bacterial Outer Membrane Proteins
Bias (Epidemiology)
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Immunity, Mucosal*
Immunization
Immunoglobulin G
Mice*
Plasmids
Poultry
Salmonella typhimurium*
Salmonella*
Bacterial Outer Membrane Proteins
Immunoglobulin G
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