Korean J Otolaryngol-Head Neck Surg.  2000 Mar;43(3):276-282.

House Dust Mite Allergic Rhinitis Model in C57BL/6 Mice

Affiliations
  • 1Department of Otorhinolaryngology, Inje University, Sanggye Paik Hospital.
  • 2Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, korea. chulhee@snu.ac.kr

Abstract

BACKGROUND AND OBJECTIVES: The animal model of allergic rhinitis is important to study the pathophysiology of allergy and to design an effective therapy to ameliorate allergic diseases. Despite of numerous reported animal models of allergic rhinitis, there were few reports of murine model sensitized with house dust mite, which is one of the most common antigen that induce allergic rhinitis. The aim of this study was the establishment of the murine model for house dust mite allergy.
MATERIALS AND METHODS
C57BL/6 mice were sensitized with Dermatophagoides farinae (Der f) crude extract in complete Freund's adjuvant and repetitive intranasal instillation of Der f extract a total of 6 times at 1 week intervals (group A). In some mice, allergen was intranasally instilled at 1 week intervals without sensitization of allergen (group B).
RESULTS
After allergen challenge, nasal symptoms were significantly increased in group A mice. Histopathologically, the number of eosinophil in nasal mucosa were also significantly increased in group A and B mice. High level of Der f-specific IgE antibody was observed in group A mice, whereas those of group B mice was low.
CONCLUSION
Our findings suggested that house dust mite allergy can be developed by systemic sensitization of Der f extract with adjuvant and intranasal instillation of allergen. This is more effective method than local sensitization of allergen only.

Keyword

Dermatophagoides farinae; Allergy animal model; Allergic rhinitis
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