Korean J Clin Pathol.  1998 Mar;18(1):51-56.

Vancomycin-resistant Enterococci: Incidence, Antimicrobial Susceptibility, and Resistance Genotypes

Affiliations
  • 1Department of Clinical Pathology, Ajou University School of Medicine, Suwon, Korea.

Abstract

BACKGROUND: Infections caused by vancomycin-resistant enterococci (VRE) are becoming increasingly prevalent throughout the world. VRE can spread by direct patient-to-patient contact as well as on the hands of personnel and contaminated environmental surfaces. The purpose of this study was to examine the incidence of VRE among total enterococci from clinical specimen and investigate the antimicrobial characteristics and resistance genotypes of isolated VRE.
METHODS
A total of 790 enterococcal isolates from patients over a period of 12 months were screened for vancomycin resistance using brain heart infusion agar plates supplemented with 6 g/mL of vancomycin. The incidence of VRE among enterococcal isolates was calculated from microbiology statistics program. Twenty three isolates of VRE were tested for minimal inhibitory concentrations (MIC) of vancomycin, penicillin, and gentamicin and resistance genotypes.
RESULTS
In the first half period, the incidence of VRE was 1.9%, and in the second half, the incidence increased to 7.7%. Thirteen strains were found to be highly resistant to vancomycin, penicillin and gentamicin (MIC, >128 g/mL). According to the direct PCR analyses, the frequency of vanB, vanC1, and vanC2 types was 13, 7, and 3 strains, respectively.
CONCLUSIONS
Continued vigilance, strict enforcement of infection control, and curtailment of vancomycin use seem to be our best approaches to controlling this increasingly important problem. For this purposes, accurate and timely detection of vancomycin-resistance and periodic investigation for incidence are essential.

Keyword

Vancomycin-resistant enterococci (VRE); Incidence; vanA; vanB; vanB2; vanC1; vanC2

MeSH Terms

Agar
Brain
Genotype*
Gentamicins
Hand
Heart
Humans
Incidence*
Infection Control
Penicillins
Polymerase Chain Reaction
Vancomycin
Vancomycin Resistance
Agar
Gentamicins
Penicillins
Vancomycin
Full Text Links
  • KJCP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr