Korean J Vet Res.  2015 Jun;55(2):81-88. 10.14405/kjvr.2015.55.2.81.

Selection and immunomodulatory evaluation of lactic acid bacteria suitable for use as canine probiotics

• Park SM
• Park HE
• Lee WK

• KMID: 1807795
• DOI: http://doi.org/10.14405/kjvr.2015.55.2.81

Abstract

This study was conducted to isolate lactic acid bacteria (LAB) from dog intestine and identify potential probiotic strains for canine use. One hundred and one LAB were isolated from feces of 20 healthy dogs. Acid, bile, and heat resistance along with adherence to Caco-2 cells and antimicrobial activity against pathogens were examined. To analyze immunomodulative effects, the production of nitric oxide (NO), TNF-alpha, and IL-1beta was measured using RAW 264.7 macrophages. Additionally, RAW BLUE cells were used to evaluate nuclear factor-kappaB (NF-kappaB) generation. Ultimately, three strains were selected as canine probiotics and identified as Lactobacillus reuteri L10, Enterococcus faecium S33, and Bifidobacterium longum B3 by 16S rRNA sequence analysis. The L10 and S33 strains showed tolerance to pH 2.5 for 2 h, 1.0% Oxgall for 2 h, and 60degrees C for 5 min. These strains also had strong antimicrobial activity against Escherichia coli KCTC 1682, Salmonella Enteritidis KCCM 12021, Staphylococcus aureus KCTC 1621, and Listeria monocytogenes KCTC 3569. All three strains exerted better immunomodulatory effects than Lactobacillus rhamnosus GG (LGG), a well-known commercial immunomodulatory strain, based on NO, NF-kappaB, IL-1beta, and TNF-alpha production. These results suggested that the three selected strains could serve as canine probiotics.

Keyword

Bifidobacterium longum; canine probiotics; Enterococcus faecium; immunomodulation; Lactobacillus reuteri

MeSH Terms

Animals
Bacteria*
Bifidobacterium
Bile
Caco-2 Cells
Dogs
Enterococcus faecium
Escherichia coli
Feces
Hot Temperature
Humans
Hydrogen-Ion Concentration
Immunomodulation
Intestines
Lactic Acid*
Lactobacillus reuteri
Lactobacillus rhamnosus
Listeria monocytogenes
Macrophages
NF-kappa B
Nitric Oxide
Probiotics*
Salmonella enteritidis
Sequence Analysis
Staphylococcus aureus
Sulfalene
Tumor Necrosis Factor-alpha
Lactic Acid
NF-kappa B
Nitric Oxide
Sulfalene
Tumor Necrosis Factor-alpha