Clin Exp Reprod Med.  2014 Sep;41(3):132-136. 10.5653/cerm.2014.41.3.132.

Observation of sperm-head vacuoles and sperm morphology under light microscope

• Park YS
• Park S
• Ko DS
• Park DW
• Seo JT
• Yang KM

• KMID: 1805496
• DOI: http://doi.org/10.5653/cerm.2014.41.3.132

Abstract

OBJECTIVE
The presence of sperm-head vacuoles has been suspected to be deleterious to the outcomes of assisted reproductive technology (ART). It is difficult to accurately distinguish morphologically abnormal sperm with vacuoles under a light microscope. This study was performed to analyze the result of the observation of sperm-head vacuoles using Papanicolaou staining under a light microscope and whether the male partner's age affects these vacuoles.
METHODS
Sperm morphology with vacuoles was evaluated using Papanicolaou staining and observed under a light microscope (400x) in 980 men. The normal morphology was divided into three categories (group A, <4% of normal morphology; group B, 4%-14% of normal morphology; and group C, >14% of normal morphology). The criteria for the sperm-head vacuoles were those given in the World Health Organization manual. For the analysis of the age factor, the participants were divided into the following groups: 26-30 years, 31-35 years, 36-40 years, 41-45 years, and 46-50 years.
RESULTS
The percentage of sperm-head vacuoles increased with normal sperm morphology (group A vs. groups B, C) (p<0.05). In the case of the age factor, a statistically significant difference was not observed across any of the age groups.
CONCLUSION
A majority of the sperm-head vacuoles showed a statistically significant difference among normal morphology groups. Therefore, we should consider the probability of the percentage of sperm-head vacuoles not increasing with age but with abnormal sperm morphology. A further study is required to clarify the effect of the sperm-head vacuoles on ART outcomes.

Keyword

Papanicolaou staining; Semen analysis; Sperm-head vacuole; Sperm morphology

MeSH Terms

Age Factors
Humans
Male
Reproductive Techniques, Assisted
Semen Analysis
Spermatozoa*
Vacuoles*
World Health Organization