J Korean Med Sci.  2010 Apr;25(4):602-607. 10.3346/jkms.2010.25.4.602.

Virulence Characteristics and Phylogenetic Background of Ciprofloxacin Resistant Escherichia coli in the Urine Samples from Korean Women with Acute Uncomplicated Cystitis

• Kim CS
• Kim ME
• Cho YH
• Cho IR
• Lee G

• KMID: 1792932
• DOI: http://doi.org/10.3346/jkms.2010.25.4.602

Abstract

To clarify the characteristics of the virulence factors (VFs) of ciprofloxacin resistant Escherichia coli (CFRE) with acute uncomplicated cystitis (AUC), we determined the VFs and the phylogenetic background of all 54 CFRE strains and the 55 randomly selected ciprofloxacin sensitive E. coli strains (CFSE) from patients with AUC in 22 Korean hospitals. The prevalence of the VFs was as follows: fimA, papEF, papGIII, sfaI, dafaBC, cnf1, and hlyA were presented in 96%, 54%, 68%, 91%, 49%, 72%, and 29% of the samples, respectively. The expressions of papEF, cnf1, and hlyA were significantly more prevalent in the CFSE. Moreover, the expressions of cnf, and papEF significantly reduced the risk of ciprofloxacin resistance. The CFSE was also marginally associated with the group B2 (P=0.05). Although the presence of pyuria and a previous cystitis history were not related with the phylotyping and the expressions of VFs, group B2, and fimA and papEF were more expressed in the younger age patients (P<0.05). In conclusion, the CFRE exhibits a selective loss of VFs and the non-B2 phylotype in Korean AUC patients. The group B2 and the presence of fimA and papEF are associated with a younger age of AUC patients.

Keyword

Ciprofloxacin; Cystitis; Virulence Factor; Escherichia coli

MeSH Terms

Adolescent
Adult
Aged
Asian Continental Ancestry Group/genetics
Bacterial Typing Techniques
*Ciprofloxacin/pharmacology/therapeutic use
*Cystitis/drug therapy/microbiology/urine
Drug Resistance, Bacterial/drug effects/*genetics
*Escherichia coli/classification/genetics/pathogenicity
Escherichia coli Infections/*drug therapy
Female
Genotype
Humans
Microbial Sensitivity Tests
Middle Aged
Phylogeny
Prospective Studies
Urine/*microbiology
Young Adult
Ciprofloxacin

Figure

• 

  Fig. 1 Examples of single PCR for detecting virulence factors (A) and multiplex PCR for phylotyping (B). M, 100 bp marker.

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