J Korean Med Sci.  2010 Dec;25(12):1716-1721. 10.3346/jkms.2010.25.12.1716.

IS6110-Restriction Fragment Length Polymorphism and Spoligotyping Analysis of Mycobacterium tuberculosis Clinical Isolates for Investigating Epidemiologic Distribution in Korea

• Choi GE
• Jang MH
• Song EJ
• Jeong SH
• Kim JS
• Lee WG
• Uh Y
• Roh KH
• Lee HS
• Shin JH
• Ryoo NH
• Kim YR
• Jeong J
• Kim JH
• Lee SM
• Yi J
• Hwang SH
• Kim HH
• Lee EY
• Chang CL
• Kim MB
• Kim YD

• KMID: 1792893
• DOI: http://doi.org/10.3346/jkms.2010.25.12.1716

Abstract

The Beijing family of Mycobacterium tuberculosis has been emerging in the world. However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates of M. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M. tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview of the distribution of genotypes of M. tuberculosis in Korea. These findings indicate that we have to pay more attention to control of M. tuberculosis strains associated with the Beijing family.

Keyword

Mycobacterium tuberculosis; IS6110-RFLP; Spoligotyping; Beijing Family; K Strain

MeSH Terms

Drug Resistance, Bacterial
Genotype
Humans
Microbial Sensitivity Tests
Mycobacterium tuberculosis/*classification/genetics/isolation & purification
Phenotype
Polymorphism, Restriction Fragment Length
Republic of Korea
Tuberculosis/*epidemiology/genetics/microbiology

Figure

• 

  Fig. 1 Results of RFLP and spoligotyping of 96 M. tuberculosis isolates. Two solid boxes indicate that the two pairs of four isolates showed identical IS6110-RFLP patterns in each pair.

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