Korean J Parasitol.  2014 Oct;52(5):507-511. 10.3347/kjp.2014.52.5.507.

First Evaluation of an Outbreak of Bovine Babesiosis and Anaplasmosis in Southern Brazil Using Multiplex PCR

• Canever MF
• Vieira LL
• Reck C
• Richter L
• Miletti LC

• KMID: 1792216
• DOI: http://doi.org/10.3347/kjp.2014.52.5.507

Abstract

Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil.

Keyword

Babesia bovis; Babesia bigemina; Anaplasma marginale; multiplex-PCR; Brazil

MeSH Terms

Anaplasma marginale/genetics/isolation & purification
Anaplasmosis/*epidemiology
Animals
Babesia/genetics/isolation & purification
Babesiosis/*epidemiology
Brazil/epidemiology
Cattle
DNA, Protozoan/blood/isolation & purification
Disease Outbreaks/*veterinary
Polymerase Chain Reaction/*veterinary
DNA, Protozoan