Korean J Parasitol.  2012 Dec;50(4):385-390.

Trichinella spiralis Infection Suppressed Gut Inflammation with CD4+CD25+Foxp3+ T Cell Recruitment

Affiliations
  • 1Department of Parasitology, Pusan National University School of Medicine, Busan 626-813, Korea. hsyu@pusan.ac.kr
  • 2Department of Microbiology and Immunology, Pusan National University School of Medicine, Busan 626-813, Korea.

Abstract

In order to know the effect of pre-existing Trichinella spiralis infection on experimentally induced intestinal inflammation and immune responses, we induced colitis in T. spiralis-infected mice and observed the severity of colitis and the levels of Th1, Th2, and regulatory cytokines and recruitment of CD4+CD25+Foxp3+ T (regulatory T; Treg) cells. Female C57BL/6 mice were infected with 250 muscle larvae; after 4 weeks, induction of experimental colitis was performed using 3% dextran sulfate sodium (DSS). During the induction period, we observed severity of colitis, including weight loss and status of stool, and evaluated the disease activity index (DAI). A significantly low DAI and degree of weight loss were observed in infected mice, compared with uninfected mice. In addition, colon length in infected mice was not contracted, compared with uninfected mice. We also observed a significant increase in production of pro-inflammatory cytokines, IL-6 and IFN-gamma, in spleen lymphocytes treated with DSS; however, such an increase was not observed in infected mice treated with DSS. Of particular interest, production of regulatory cytokines, IL-10 and transforming growth factor (TGF)-beta, in spleen lymphocytes showed a significant increase in mice infected with T. spiralis. A similar result was observed in mesenteric lymph nodes (MLN). Subsets of the population of Treg cells in MLN and spleen showed significant increases in mice infected with T. spiralis. In conclusion, T. spiralis infection can inhibit the DSS-induced colitis in mice by enhancing the regulatory cytokine and Treg cells recruitment.

Keyword

Trichinella spiralis; dextran sulfate sodium (DSS); CD4+CD25+Foxp3+ T (Treg) cells; intestinal inflammation

MeSH Terms

Animals
Colitis/chemically induced/*immunology/pathology
Cytokines/genetics/*immunology/metabolism
Dextran Sulfate/adverse effects
Disease Models, Animal
Female
Forkhead Transcription Factors/immunology/metabolism
*Gene Expression Regulation
Larva
Mice
Mice, Inbred C57BL
Spleen/immunology
T-Lymphocytes, Regulatory/*immunology
Trichinella spiralis/*immunology
Trichinellosis/*immunology/parasitology
Cytokines
Dextran Sulfate
Forkhead Transcription Factors
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