Korean J Physiol Pharmacol.  2015 May;19(3):197-202. 10.4196/kjpp.2015.19.3.197.

Effect of Sulfonylureas Administered Centrally on the Blood Glucose Level in Immobilization Stress Model

• Sharma N
• Sim YB
• Park SH
• Lim SM
• Kim SS
• Jung JS
• Hong JS
• Suh HW

• KMID: 1791419
• DOI: http://doi.org/10.4196/kjpp.2015.19.3.197

Abstract

Sulfonylureas are widely used as an antidiabetic drug. In the present study, the effects of sulfonylurea administered supraspinally on immobilization stress-induced blood glucose level were studied in ICR mice. Mice were once enforced into immobilization stress for 30 min and returned to the cage. The blood glucose level was measured 30, 60, and 120 min after immobilization stress initiation. We found that intracerebroventricular (i.c.v.) injection with 30 microg of glyburide, glipizide, glimepiride or tolazamide attenuated the increased blood glucose level induced by immobilization stress. Immobilization stress causes an elevation of the blood corticosterone and insulin levels. Sulfonylureas pretreated i.c.v. caused a further elevation of the blood corticosterone level when mice were forced into the stress. In addition, sulfonylureas pretreated i.c.v. alone caused an elevation of the plasma insulin level. Furthermore, immobilization stress-induced insulin level was reduced by i.c.v. pretreated sulfonylureas. Our results suggest that lowering effect of sulfonylureas administered supraspinally against immobilization stress-induced increase of the blood glucose level appears to be primarily mediated via elevation of the plasma insulin level.

Keyword

Blood glucose; Brain; Immobilization stress; Sulfonylurea

MeSH Terms

Animals
Blood Glucose*
Brain
Corticosterone
Glipizide
Glyburide
Immobilization*
Insulin
Mice
Mice, Inbred ICR
Plasma
Tolazamide
Blood Glucose
Corticosterone
Glipizide
Glyburide
Insulin
Tolazamide