J Korean Med Sci.  2011 Jun;26(6):720-725. 10.3346/jkms.2011.26.6.720.

Effects of Intronic and Exonic Polymorphisms of Paraoxonase 1 (PON1) Gene on Serum PON1 Activity in a Korean Population

• Eom SY
• Kim YS
• Lee CJ
• Lee CH
• Kim YD
• Kim H

• KMID: 1785955
• DOI: http://doi.org/10.3346/jkms.2011.26.6.720

Abstract

Paraoxonase 1 (PON1) hydrolyzes a number of toxic organophosphorous compounds and reduces lipid peroxide accumulation, and PON1 genetic polymorphisms in the coding region modulate serum PON1 activity. In this study, we investigated the association between 3 polymorphisms of PON1 located in intron 5 (17899insdelTT and 17974CT) and exon 6 (192QR) and serum PON1 activity. The genetic polymorphisms and serum activity of PON1 were analyzed in 153 healthy Koreans by using a direct sequencing assay and spectrophotometric method, respectively. A significant linkage disequilibrium (LD) was observed between all tested single nucleotide polymorphisms, with the strongest LD observed between 17899insdelTT and 192QR (D' = 0.984). The 17899insdelTT, 17974CT and 192QR genetic polymorphisms were associated with significant differences in serum paraoxonase activity. In multiple regression analyses, smoking, triglyceride level, high-density lipoprotein (HDL) level, and the 17899insdelTT and 192QR genetic polymorphisms were significant determinants of serum paraoxonase activity, while age, smoking, triglyceride level, HDL level, and the 192QR genetic polymorphism were significant determinants of serum arylesterase activity. These results suggest that although the 192QR genetic polymorphism in the coding region of PON1 is primarily associated with serum PON1 activity, the intronic polymorphisms are also involved in serum PON1 activity, and this association may be mediated by LD.

Keyword

Paraoxonase 1 (PON1); Genetic Polymorphism; Linkage Disequilibrium; serum PON1 activity

MeSH Terms

Aged
Alleles
Aryldialkylphosphatase/blood/*genetics
Asian Continental Ancestry Group/*genetics
Exons
Female
Gene Frequency
Genotype
Humans
Introns
Linkage Disequilibrium
Lipoproteins, HDL/blood
Male
Middle Aged
*Polymorphism, Genetic
Polymorphism, Single Nucleotide
Regression Analysis
Republic of Korea
Smoking
Triglycerides/blood

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