J Korean Med Sci.  2007 Dec;22(6):943-945. 10.3346/jkms.2007.22.6.943.

Mechanisms of Epithelial-Mesenchymal Transition of Peritoneal Mesothelial Cells During Peritoneal Dialysis

Affiliations
  • 1Hyonam Kidney Laboratory, Soon Chun Hyang University, Seoul, Korea.
  • 2College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University, Seoul, Korea. hha@ewha.ac.kr

Abstract

A growing body of evidence indicates that epithelial-mesenchymal transition (EMT) of human peritoneal mesothelial cells (HPMC) may play an important role in the development and progression of peritoneal fibrosis during long-term peritoneal dialysis (PD) leading to failure of peritoneal membrane function. Here, we review our own observations and those of others on the mechanisms of EMT of HPMC and suggest potential therapeutic strategies to prevent EMT and peritoneal fibrosis during long-term PD. We found that high glucose and H2O2 as well as transforming growth factor-beta1 (TGF-beta1) induced EMT in HPMC and that high glucoseinduced EMT was blocked not only by inhibition of TGF-beta1 but also by antioxidants or inhibitors of mitogen-activated protein kinases (MAPK). Since MAPKs are downstream target molecules of reactive oxygen species (ROS), these data suggest that high glucose-induced generation of ROS and subsequent MAPK activation mediate high glucose-induced EMT in HPMC. We and others also observed that bone morphogenetic protein-7 (BMP-7) prevented EMT in HPMC. Glucose degradation products (GDP) were shown to play a role in inducing EMT. Involvement of a mammalian target of rapamycin (mTOR) in TGF-beta1-induced EMT has also been proposed in cultured HPMC. A better understanding of the precise mechanisms involved in EMT of HPMC may provide new therapeutic strategies for inhibiting peritoneal fibrosis in long-term PD patients.

Keyword

Bone Morphogenetic Protein-7; Fibrosis; Mitogen-Activated Protein Kinases; Reactive Oxygen Species; Peritoneal Dialysis

MeSH Terms

Epithelial Cells/*pathology
Fibrosis
Humans
Mesoderm/*pathology
Peritoneal Dialysis/*adverse effects
Peritoneum/*pathology

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