J Korean Acad Nurs.  2012 Jun;42(3):443-451. 10.4040/jkan.2012.42.3.443.

A Comparison of the Rates of Hemolysis and Repeated Blood Sampling using Syringe needles versus Vacuum tube needles in the Emergency Department

Affiliations
  • 1Department of Clinical Nursing Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
  • 2Department of Nursing Science, School of Oriental Medicine in WooSuk University, Jeonbuk, Korea. msyellow45@hanmail.net
  • 3Department of Nurisng, Samsung Medical Center, Seoul, Korea.
  • 4Department of Laboratory & Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
  • 5Department of Laboratory & Genetics, Samsung Medical Center, Seoul, Korea.
  • 6School of Nursing, University of Maryland, Baltimore, USA.

Abstract

PURPOSE
This study was done to compare the rates of hemolysis and repeated sampling in blood samples obtained by a syringe needle versus a vacuum tube needle.
METHODS
A randomized, prospective study was used to evaluate the differences between the two blood sampling methods. The study group consisted of patients seen in the emergency department (ED) for blood sampling to determine electrolyte level. ED patients were randomly assigned to either the syringe group or the vacuum tube group. All blood samples were collected by experienced ED nurses and hemolysis was determined by experienced laboratory technologists. Data were analyzed using Fisher's exact test and binary logistic regression.
RESULTS
One hundred forty-five valid samples were collected (74 in the syringe group versus 71 in the vacuum tube group). 5 of 74 (6.8%) blood samples in the syringe group and 8 of 71 (11.3%) in the vacuum tube group hemolyzed. Repeated blood sampling occurred for 2 of 74 (2.7%) and 3 of 71 (4.2%) in each group respectively. There were no significant differences in rates of hemolysis and repeated sampling between two groups (B=1.97, p=.204; B=2.36, p=.345).
CONCLUSION
Venipuncture with syringe needles can be recommended for ED nurses to obtain blood samples.

Keyword

Hemolysis; Blood specimen collection; Syringe; Vacuum tube; Emergency

MeSH Terms

Adult
Aged
Blood Specimen Collection/instrumentation/*methods
Emergency Service, Hospital
Female
*Hemolysis
Humans
Logistic Models
Male
Middle Aged
Phlebotomy
Prospective Studies
Questionnaires
Syringes

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