J Periodontal Implant Sci.  2010 Apr;40(2):61-68. 10.5051/jpis.2010.40.2.61.

Identification of immunological parameters associated with the alveolar bone level in periodontal patients

• Park CS
• Lee JY
• Kim SJ
• Choi JI

• KMID: 1783536
• DOI: http://doi.org/10.5051/jpis.2010.40.2.61

Abstract

PURPOSE
The present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum IgG titer against periodontopathogenic bacteria, the expression of the helper T-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (HSP) epitope peptide from P. gingivalis HSP60.
METHODS
Twenty-five patients with moderate periodontitis had their gingival connective tissue harvested of gingival mononuclear cells during an open flap debridement procedure and peripheral blood was drawn by venipuncture to collect serum. The mean level of interproximal alveolar bone was calculated to be used as an index for periodontal disease severity for a given patient. Each of selected immunologic parameters was subject to statistical management to seek their correlations with the severity of periodontal disease.
RESULTS
A significant correlation could not be identified between serum IgG titers against specific bacteria (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Streptococcus mutans) and the severity of periodontal disease. Expression of interleukin (IL)-10 by gingival mononuclear cells was statistically significant in the group of patients who had higher levels of alveolar bone height. However, a similar correlation could not be demonstrated in cases for IL-4 or interferon-gamma. Patients' serum reactivity to cross-reactive epitope peptide showed a significant correlation with the amount of alveolar bone.
CONCLUSIONS
It was concluded that expression of IL-10 by gingival mononuclear cells and patients' sero-reactivity to the cross-reactive HSP peptide of P. gingivalis HSP60 were significantly correlated with alveolar bone height.

Keyword

Heat-shock proteins; Immunoglobulin G; T-Lymphocytes

MeSH Terms

Actinobacillus actinomycetemcomitans
Bacteria
Connective Tissue
Debridement
Fusobacterium nucleatum
Heat-Shock Proteins
Humans
Immunoglobulin G
Interferon-gamma
Interleukin-10
Interleukin-4
Interleukins
Periodontal Diseases
Periodontitis
Phlebotomy
Prevotella intermedia
Streptococcus
T-Lymphocytes
T-Lymphocytes, Helper-Inducer
Heat-Shock Proteins
Immunoglobulin G
Interferon-gamma
Interleukin-10
Interleukin-4
Interleukins

Figure

• 

  Figure 1 Expression pattern of helper T-cell cytokine (interleukin [IL]-10, interferon gamma [IFN-γ], IL-4) by gingival tissues obtained from 25 periodontal patients.

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  Figure 2 Dot immunoblot patterns of serum to peptide #19 from P. gingivalis heat shock protein (HSP, pg#19) and human HSP60 (hu#19), respectively, in Group A and B patients.

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