J Korean Acad Periodontol.  2009 Mar;39(1):45-52. 10.5051/jkape.2009.39.1.45.

The biologic effect of fibrin-binding synthetic oligopeptide on periodontal ligament cells

• Lee JA
• Ku Y
• Park YJ
• Koo KT
• Kim TI
• Seol YJ
• Lee YM
• Rhyu IC
• Chung CP

• KMID: 1783516
• DOI: http://doi.org/10.5051/jkape.2009.39.1.45

Abstract

PURPOSE: Fibronectin(FN), one of the major components of ECM, mediates wide variety of cellular interactions including cell adhesion, migration, proliferation and differentiation. In this study, we used synthetic peptides based on fibrin binding sites of amino-terminal of FN and evaluated their biologic effects on periodontal ligament(PDL) cells.
MATERIALS AND METHODS
PDL cells were cultured on synthetic oligopeptides coated dishes and examined for cell adhesion, proliferation via confocal microscope. For detection of ERK1/2, cells were plated and Western blot analysis was performed.
RESULTS
PDL cells on synthetic oligopeptide coated dishes showed enhanced cell adhesion and proliferation. Western blot analysis revealed increased level of ERK1/2 phosphorylation in cells plated on FN fragment containing fibrin-binding domain(FF1 and FF5) coated dishes.
CONCLUSION
These results reveals that FN fragment containing fibrin-binding domain possess an enhanced biologic effect of PDL ligament cells.

Keyword

Fibronectin; periodontal ligament cells; fibrin-binding domain; ERK1/2

MeSH Terms

Binding Sites
Blotting, Western
Cell Adhesion
Fibrin
Fibronectins
Ligaments
Oligopeptides
Peptides
Periodontal Ligament
Phosphorylation
Fibrin
Fibronectins
Oligopeptides
Peptides

Figure

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  Figure 1 PDL cell attachment using confocal laser microscopy. Nuclei appear blue, and actin filaments are red. (A) NT, (B) FF1, (C) FF3, (D) FF5

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  Figure 2 MTT assay of PDL cell attachment in 1 hour. *denotes a statistically significant differences between control and test group (P<0.05). †denotes statistically significant differences between test groups(P<0.05).

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  Figure 3 Activation of ERK1/2 in PDL cells cultured on synthetic oligopeptide. Phosphorylation levels of p-ERK1/2 were quantified by densitometry. Immunoblotting after treatment with 20µM of each peptide. (A) Phosphorylated ERK1/2, (B) β-actin, (C) The levels of p-ERK1/2 were quantified by densitometric scanning

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