Infect Chemother.  2008 Jun;40(3):167-169. 10.3947/ic.2008.40.3.167.

Comparison of Real-time PCR Methods and pp65 Antigenemia Assay to Detect Cytomegalovirus Reactivation in Hematopoietic Stem Cell Transplantation

• Lee SY
• Choi BS
• Kim SS
• Choi SM
• Shin WS
• Lee JS

• KMID: 1782290
• DOI: http://doi.org/10.3947/ic.2008.40.3.167

Abstract

Human cytomegalovirus (HCMV) is a common human pathogen that causes morbidity and mortality in hematopoietic stem cell transplantation (HSCT) recipients. Early diagnosis of HCMV infection or reactivation, and setting threshold values for effective pre-emptive therapies, are required for appropriate HCMV disease prevention in HSCT recipients. We compared the HCMV infections detected by the two methods, LightCycler-based PCR (LC PCR) and in-house immediate early protein PCR (in-house IE PCR) with the results of a pp65 antigenemia assay as the reference. The sensitivity and specificity for the in-house IE PCR were 79.3% and 72.7%, respectively, and 82.9% and 40.7%, respectively, for the LC PCR. The correlation between the HCMV viral load and pp65 antigenemia in HSCT recipients was r=0.603 with in-house IE PCR and r=0.525 with LC PCR. The discordant results between methods and relatively low (r) values suggest that we need more study to set threshold values according to the using methods with clinical outcome.

Keyword

HCMV; Real-time PCR; pp65 antigenemia; LC PCR; In-house IE PCR

MeSH Terms

Cytomegalovirus
Early Diagnosis
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Viral Load