J Korean Med Sci.  2006 Apr;21(2):304-308. 10.3346/jkms.2006.21.2.304.

The Effect of Vero Cell Coculture on the Development of Mouse Embryos Exposed to Monoclonal Antibodies Specific for Mammalian Heat Shock Protein 60

• Noh JH
• Chung KN
• Kim YB

• KMID: 1781840
• DOI: http://doi.org/10.3346/jkms.2006.21.2.304

Abstract

Heat shock proteins (HSP) have been identified as an important factor of a very complex and highly conserved cellular defense mechanism to preserve cell survival under adverse environmental conditions. HSP 60 are immunodominant antigens of microbe such as Chlamydia trachomatis and have a potentiality to become a target antigen due to antigenic similarity between chlamydial and human HSP. This study was conducted to investigate the effects of Vero cell coculture to anti-HSP 60 on the early mouse embryo development in vitro. The 2-cell mouse embryos (ICR) were cultured and mouse embryo development was observed every 24 hr for 3 days. 45% and 22.1% of the embryos cultured in Ham's F-10 plus anti HSP 60 with Vero cells developed to the 4- to 8- cell stage (day 1) and morular stage (day 2) as compared with 29.2% and 2.7% of those cultured without Vero cells respectively. But at day 3, the beneficial effect of Vero cells was not noted. These findings suggest that Vero cells have some roles to overcome the detrimental effect of anti-HSP 60 to some degree. These results suggest that Vero cells coculture will promote reproductive outcome in patient previously sensitized to microbial (e.g. Chlamydia trachomatis) HSP 60.

Keyword

Heat-Shock Proteins; Anti-Heat Shock Proteins 60; Mice; Embryo; Vero Cells; Coculture Techniques

MeSH Terms

Vero Cells
Pregnancy
Mice, Inbred ICR
Mice
Male
Infertility, Female/etiology/immunology/therapy
Immunodominant Epitopes
Female
Embryonic Development/*immunology
Coculture Techniques
Chlamydia trachomatis/immunology/pathogenicity
Chaperonin 60/*immunology
Cercopithecus aethiops
Antigens, Bacterial
Antibodies, Monoclonal/*administration & dosage
Animals

Figure

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  Fig. 1 Two-cell stage mouse embryos were collected at 48 hr after hCG injection from oviducts of mated ICR mice (×200).

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  Fig. 2 Photographs of the mouse embryos in vitro cultured for 24 hr in media containing anti-HSP 60 (100 µg/mL) with Vero cell coculture (A), and containing anti-HSP 60 without Vero cell coculture (B) (×200).

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  Fig. 3 Photographs of the mouse embryos in vitro cultured for 48 hr in media containing anti-HSP 60 (100 µg/mL) with Vero cell coculture (A), and containing anti-HSP 60 without Vero cell coculture (B) (×200).

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  Fig. 4 Photographs of the mouse embryos in vitro cultured for 72 hr in media containing anti-HSP 60 with Vero cell coculture (×200).

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  Fig. 5 Effect of Vero cell coculture on the development of 2-cell mouse embryos exposed to anti HSP-60 after 24, 48, 72 hr in culture respectively.

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