Korean J Lab Med.  2008 Feb;28(1):24-33. 10.3343/kjlm.2008.28.1.24.

Identification of Mycobacteria Species by HPLC and Species Distribution during Five Years at Ulsan University Hospital

• Jeong J
• Kim SR
• Chang CL
• Lee SH

• KMID: 1781555
• DOI: http://doi.org/10.3343/kjlm.2008.28.1.24

Abstract

BACKGROUND: Infections caused by mycobacteria have been significantly increasing. Due to the difficulty of making a decision about the pathogenicity of mycobacteria, species-level identification is very important for patients' diagnosis and treatment. The purpose of this study was to identify mycobacteria species using a high performance liquid chromatography (HPLC) method and to provide an initial database for the distribution of mycobacteria in Korea.
METHODS
Acid fast bacteria isolated from 3,107 clinical specimens were identified by mycolic acid analysis using HPLC. The HPLC patterns were compared with those of standard mycobacteria species.
RESULTS
The HPLC patterns were divided into single, double, and triple cluster groups, each group comprising 9, 20, and 4 species, respectively. Mycobacteria and non-tuberculous mycobacteria (NTM) were identifies by HPLC at the rates of 99.5% and 95.6%, respectively. NTM was isolated in 12.4% of the mycobacteria positive specimens. This study also found that there were 20 different NTM species with the distribution of each species ranging from 0.3% to 15.9% of the total NTM. While the rate of NTM has been increasing in Korea, M. avium-intracellulare, M. fortuitum, and M. chelonae are relatively decreasing, and M. kansasii and M. gordonae are relatively increasing.
CONCLUSIONS
HPLC method was highly discriminative for the identification of NTM in clinical specimens.

Keyword

Mycobacteia; M. tuberculosis; Nontuberculous mycobacteria; HPLC

MeSH Terms

Bacterial Typing Techniques
Chromatography, High Pressure Liquid/*methods
Hospitals, University
Humans
Korea
Mycobacteria, Atypical/chemistry/*isolation & purification
Mycobacterium Infections, Atypical/drug therapy/microbiology
Mycolic Acids/analysis

Figure

• 

  Fig. 1. Characteristic HPLC chromatograms of standard Mycobacterium species with single-cluster peak patterns. (A) M. asiaticum. (B) M. bovis. (C) M. gastri. (D) M. gordonae. (E) M. kansasii. (F) M. marinum. (G) M. szulgai. (H) M. trivial and (I) M. tuberculosis.

• 

  Fig. 2. Characteristic HPLC chromatograms of standard Mycobacterium species with double-cluster peak patterns. (A) M. abscessus. (B) M. acapulsensis. (C) M. agri. (D) M. avium. (E) M. celatum. (F) M. chelonae. (G) M. diernhoferi. (H) M. flavescens. (I) M. fortuitum. (J) M. gilvum. (K) M. intracellulare. (L) M. mucogenicum. (M) M. nonchromogenicum. (N) M. peregrinum. (O) M. phlei. (P) M. porcinum. (Q) M. scrofulaceum. (R) M. smegmatis. (S) M. terrae and (T) M. xenopi.

• 

  Fig. 3. Characteristic HPLC chromatograms of standard Mycobacterium species with triple-cluster peak patterns. (A) M. austroafricanum. (B) M. pulveris. (C) M. simiae and (D) M. vaccae.

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