Korean J Lab Med.  2007 Dec;27(6):458-463. 10.3343/kjlm.2007.27.6.458.

Development of a Web-based Program for the Identification of Human Leukocyte Antigen Antibody Specificities

Affiliations
  • 1Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea. hboh@amc.seoul.kr
  • 2Department of Computer Science Education, College of Education, Korea University, Seoul, Korea.

Abstract

BACKGROUND: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but it is very difficult to perform manually. METHODS: To develop a web-based program, PHP (5.1.2), Apache (2.0.55), and MySQL (5.0.22) were used. Tail analysis algorithm was applied to identify specificities, which analyzed statistically 2 x 2 tables representing reactivities to broad antigens, splits and cross reactive groups (CREG). Exploiting two CREG classifications of Rodey (R) and Takemoto (T), antibody specificities were identified by 3 methods (ABC, R-ABC, T-ABC) simultaneously. Performance of the system was evaluated using 159 samples that showed > or =6 PRA% by a lymphocytotoxicity assay. RESULTS: A web-based system that can identify HLA antibody specificities was implemented on www.koreanhla.com. Among 159 samples tested, antibody specificities were identified in 151 (95.0 %), but not in 8 samples with PRA >97%. Among the 151 samples, 110 showed broad or split specificities and 41 CREG specificities. CONCLUSIONS: We developed a web-based computer program for the identification of HLA antibody specificities. Accessible to everyone on the internet, this program should be of help in sharing PRA results among laboratories.

Keyword

Panel reactive antibody (PRA); Tail analysis; Human leukocyte antigens (HLA); Antibody specificity

MeSH Terms

Algorithms
*Antibody Specificity/genetics
HLA Antigens/genetics/*immunology
Histocompatibility Testing
Humans
Internet
*Software

Figure

  • Fig. 1. The input screen of the computer program.

  • Fig. 2. The output screen of the computer program.


Cited by  1 articles

Experience on Identification of Cross-Reactive Group Specificity Performed by Anti-human Globulin Panel Reactive Antibody Tests
Yong-Hak Sohn, Choong-Hwan Cha, Myeong Hee Kim, Sun-Young Ko, Heung-Bum Oh
Korean J Lab Med. 2008;28(5):362-370.    doi: 10.3343/kjlm.2008.28.5.362.


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