Korean J Lab Med.  2007 Jun;27(3):162-168. 10.3343/kjlm.2007.27.3.162.

Evaluation of the Abbott Cell-Dyn Sapphire Hematology Analyzer

• Park Y
• Song J
• Song S
• Song KS
• Ahn MS
• Yang MS
• Kim I
• Choi JR

• KMID: 1781478
• DOI: http://doi.org/10.3343/kjlm.2007.27.3.162

Abstract

BACKGROUND: The performance of Cell-Dyn Sapphire (Abbott Diagnostic, USA) was compared to the Bayer Advia 2120 (Bayer Diagnostics, USA), Sysmex XE-2100 (Sysmex Corporation, Japan), and reference microscopy. METHODS: Three hundred samples for routine CBC and WBC differentials were randomly chosen for a comparison analysis. The Cell-Dyn Sapphire system was evaluated according to the linearity, imprecision, inter-instrument correlations, and white blood cell differential. RESULTS: The CBC parameters (WBC, RBC, hemoglobin and platelet) showed a significant linearity with correlation coefficients greater than 0.99 (P<0.0001). Coefficients of variation (CV) for withinrun and differential count of WBC were less than 5% except for Total CV for monocytes, eosinophils, and basophils and within-run CV for low valued eosinophils. The correlation coefficients with manual count were lower in monocytes, eosinophils, and basophils than in neutrophils and lymphocytes. The correlation with other hematology anlayzers was significant exclusive of basophils. CONCLUSIONS: These results demonstrate that the Cell-Dyn Sapphire has a good linearity, an acceptable reproducibility, a minimal carryover, and a comparable performance with the sysmex XE-2100 and Advia 2120.

Keyword

Cell-Dyn Sapphire; Performance; Correlation

MeSH Terms

Analysis of Variance
Autoanalysis
Blood Cell Count/*instrumentation/methods
Blood Specimen Collection
Diagnostic Errors
Humans
Reproducibility of Results
Sensitivity and Specificity

Figure

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  Fig. 1. Linearities of WBC count, platelet count, RBC count, and hemoglobin analyzed by CD-Sapphire.

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  Fig. 2. Inter-instrument comparison of reticulocyte (RETC) and immature reticulocyte fraction (IRF). Advia 2120 data correspond to combined proportions of medium and high staining reticulocytes.

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  Fig. 3. Comparisons of CD-Sapphire (optical), Advia 2120 (optical), CD-Sapphire immunoplatelet (CD61), and Flow cytometry (Coulter FC500) for platelet count (×109/L).

Reference

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