Ann Lab Med.  2012 Jan;32(1):79-81. 10.3343/alm.2012.32.1.79.

Evaluation of a New Immunochromatographic Assay Kit for the Rapid Detection of Norovirus in Fecal Specimens

• Park KS
• Baek KA
• Kim DU
• Kwon KS
• Bing SH
• Park JS
• Nam HS
• Lee SH
• Choi YJ

• KMID: 1781442
• DOI: http://doi.org/10.3343/alm.2012.32.1.79

Abstract

Rapid and accurate detection of norovirus is essential for the prevention and control of norovirus outbreaks. This study compared the effectiveness of a new immunochromatographic assay kit (SD BIOLINE Norovirus; Standard Diagnostics, Korea) and real-time reverse transcription-PCR (RT-PCR) for detecting norovirus in fecal specimens. Compared with real-time RT-PCR, the new assay had sensitivity, specificity, positive predictive value, and negative predictive value of 76.5% (52/68), 99.7% (342/343), 98.1% (52/53), and 95.5% (342/358), respectively. The sensitivity of the assay was 81.8% (18/22) for GII.3 and 75.7% (28/37) for GII.4. None of the 38 enteric virus-positive specimens (3 for astrovirus, 5 for enteric adenovirus, and 30 for rotavirus) tested positive in the cross-reactivity test performed by using this assay. The new immunochromatographic assay may be a useful screening tool for the rapid detection of norovirus in sporadic and outbreak cases; however, negative results may require confirmatory assays of greater sensitivity.

Keyword

Immunochromatographic assay; Norovirus; Sensitivity; Specificity

MeSH Terms

Acute Disease
Adolescent
Adult
Aged
Aged, 80 and over
Caliciviridae Infections/*diagnosis
Child
Child, Preschool
Feces/*virology
Gastroenteritis/*diagnosis/virology
Humans
*Immunoassay
Infant
Middle Aged
Norovirus/*genetics/isolation & purification
RNA, Viral/analysis
Reagent Kits, Diagnostic
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity

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