J Korean Med Sci.
2009 Jan;24(Suppl 1):S135-S142. 10.3346/jkms.2009.24.S1.S135.
Clinical Significance of Monitoring Circulating CD4+CD25+ Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period
- Affiliations
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- 1Department of Internal Medicine, Chung-Ang University, Seoul, Korea.
- 2Department of Laboratory Medicine, The Catholic University of Korea, Seoul, Korea.
- 3Catholic Transplantation Research Center, The Catholic University of Korea, Seoul, Korea.
- 4Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea. yangch@catholic.ac.kr
- 5Department of Surgery, The Catholic University of Korea, Seoul, Korea.
- KMID: 1778153
- DOI: http://doi.org/10.3346/jkms.2009.24.S1.S135
Abstract
- The CD4+CD25+ T regulatory cells (Tregs) play an important role in immune tolerance in experimental transplantation but the clinical significance of circulating Tregs in the peripheral blood is undetermined. In 50 kidney transplant (KT) recipients, 29 healthy controls and 32 liver transplant (LT) recipients, the frequency of Tregs was measured with flow cytometry before and after transplantation. In the KT recipients, IL-10 secretion was measured with an enzyme-linked immunospot (ELISPOT) assay. The median frequency of circulating Tregs before KT was similar to that in healthy controls but significantly lower than that in LT patients before transplantation. The frequency of Tregs was significantly decreased in patients with subclinical acute rejection compared with those without subclinical acute rejection. Calcineurin inhibitors (CNIs) and anti-CD25 antibody decreased the frequency of Tregs but mTOR inhibitor did not. The frequency of donor-specific IL-10 secreting cells did not correlate with the number of Tregs. The frequency of circulating Tregs in KT recipients is strongly affected by CNIs and anti-CD25 antibody, and a low frequency of Tregs is associated with subclinical acute rejection during the early posttransplant period.
Keyword
MeSH Terms
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Adult
CD4-Positive T-Lymphocytes/*immunology
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Graft Rejection
Humans
Interleukin-10/metabolism
Interleukin-2 Receptor alpha Subunit/*biosynthesis
Kidney Failure, Chronic/blood/immunology/*therapy
Kidney Transplantation/*methods
Male
Middle Aged
Nephrology/*methods
T-Lymphocytes, Regulatory/*immunology
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