Korean J Gastroenterol.  2010 Jan;55(1):33-45. 10.4166/kjg.2010.55.1.33.

Saccharomyces boulardii Reduced Intestinal Inflammation in Mice Model of 2,4,6-trinitrobencene Sulfonic Acid Induced Colitis: Based on Microarray

• Lee SK
• Kim HJ
• Chi SG

• KMID: 1775924
• DOI: http://doi.org/10.4166/kjg.2010.55.1.33

Abstract

BACKGROUND/AIMS
Saccharomyces boulardii has been reported to be beneficial in the treatment of inflammatory bowel disease. The aim of this work was to evaluate the effect of S. boulardii in a mice model of 2,4,6-trinitrobencene sulfonic acid (TNBS) induced colitis and analyze the expression of genes in S. boulardii treated mice by microarray.
METHODS
BALB/c mice received TNBS or TNBS and S. boulardii treatment for 4 days. Microarray was performed on total mRNA form colon, and histologic evaluation was also performed.
RESULTS
In mice treated with S. boulardii, the histological appearance and mortality rate were significantly restored compared with rats receiving only TNBS. Among 330 genes which were altered by both S. boulardii and TNBS (>2 folds), 193 genes were down-regulated by S. boulardii in microarray. Most of genes which were down-regulated by S. bouardii were functionally classified as inflammatory and immune response related genes.
CONCLUSIONS
S. boulardii may reduce colonic inflammation along with regulation of inflammatory and immune responsive genes in TNBS-induced colitis.

Keyword

Saccharomyces boulardii; Microarray; TNBS colitis

MeSH Terms

Animals
Colitis/chemically induced/genetics/*therapy
Colon/metabolism/pathology
Gene Expression Profiling
Mice
Mice, Inbred BALB C
Oligonucleotide Array Sequence Analysis
*Probiotics
*Saccharomyces
Trinitrobenzenesulfonic Acid