Korean J Ophthalmol.  1995 Jun;9(1):32-46. 10.3341/kjo.1995.9.1.32.

The effects on inhibition of corneal neovascularization after human amniotic membrane transplantation in severely damaged rabbit corneas

• Kim JC
• Tseng SC

• KMID: 1735135
• DOI: http://doi.org/10.3341/kjo.1995.9.1.32

Abstract

Human amniotic membrane isolated from the placenta contained basement membrane components such as type IV collagen, laminin, and 6 and 4 integrins, all of which remained detectable while preserved in glycerin for one week. One month after the n-heptanol removal of the total corneal epithelium and the limbal lamellar keratectomy, all rabbit eyes carried features of limbal deficiency, including conjunctival epithelial ingrowth, vascularization and chronic inflammation. Ten control eyes then received a total keratectomy, and 13 experimental eyes received an additional amniotic membrane transplantation. Three-month follow-ups revealed that all control corneas were revascularized to the center with granuloma and retained a conjunctival phenotype. In contrast, in the experimental groups, 5 corneas became clear with either minimal or no vascularization; the rest had either mild peripheral (5) or total (3) vascularization and more cloudy stroma. Using monoclonal antibodies for epithelial markers and matrix components, we concluded that the success correlated with the return of a cornea-like epithelial phenotype and the preservation of the amniotic membrane, whereas the failure maintained a conjunctival epithelial phenotype and the amniotic membrane was either partially degraded or covered by host fibrovascular stroma. Measures taken to facilitate the former might prove this procedure clinically useful for ocular surface reconstruction.

Keyword

amniotic membrane; vascularization; phenotype; ocular surfacereconstruction

MeSH Terms

Amnion/chemistry/*transplantation
Animals
Antibodies, Monoclonal
Basement Membrane/chemistry/pathology
Cornea/pathology/*surgery
Corneal Neovascularization/pathology/*prevention & control/surgery
Epithelium/pathology/surgery
Extracellular Matrix Proteins/analysis
Female
Fluorescent Antibody Technique
Humans
Immunophenotyping
Male
Rabbits
Antibodies, Monoclonal
Extracellular Matrix Proteins