Korean J Ophthalmol.  1995 Jun;9(1):12-18. 10.3341/kjo.1995.9.1.12.

Safety of intravitreally injected ciprofloxacin in phakic rabbit eyes

• Kim SH
• Kim JH
• Cho KS
• Kwak JS

• KMID: 1735132
• DOI: http://doi.org/10.3341/kjo.1995.9.1.12

Abstract

This study was designed to determine the maximal safe drug concentration of intravitreal ciprofloxacin in phakic rabbit eyes. Twenty-two eyes of New Zealand pigmented rabbits received midvitreal ciprofloxacin of 100, 200, 400, 600 or 800 microgram in BSS Plus, or BSS Plus only. Retinal toxicity was dose-dependent as determined with electroretinography, light microscopy, and transmission electron microscopy. At a dose of greater than 400 microgram, disorganization of the outer segments was a main pathological finding in transmission electron microscopy. We evaluated retinal function by measuring the electroretinograms for a graded series of flash intensities and by fitting electroretinogram b-wave amplitudes to the Naka-Rushton equation. At a dose of greater than 600 microgram, Rmax was significantly decreased and log K was significantly increased. N-value tended to decrease. A decrease of b-wave amplitudes caused by retinal toxicity could be detected very sensitively with lower luminance stimuli. Determination of retinal toxicity with lower luminance electroretinography revealed a significant decrease of b-wave amplitudes at a dose of greater than 400 microgram. We concluded that a safe dose of intravitreal ciprofloxacin in phakic rabbit eyes was 200 microgram in phakic eyes.

Keyword

intravitreal ciprofloxacin; electroretinogram; Naka-Rushton equation parameters; electron microscory; retinal toxicity

MeSH Terms

Animals
Ciprofloxacin/administration & dosage/*toxicity
Dose-Response Relationship, Drug
Electroretinography/drug effects
Injections
Lens, Crystalline
Photic Stimulation
Rabbits
Retina/*drug effects/pathology/physiopathology
Rod Cell Outer Segment/drug effects/pathology
Vitreous Body
Ciprofloxacin