Yonsei Med J.  2013 May;54(3):603-608. 10.3349/ymj.2013.54.3.603.

A Novel 5'-Uncoding Region -1248 A>G Variation of Mitofusin-2 Gene Is Associated with Hypertension in Chinese

Affiliations
  • 1Department of Hypertension, Beijing Institute of Heart, Lung, Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, China. wangzg70@gmail.com, wzg70@hotmail.com
  • 2Department of Epidemiology, Beijing Institute of Heart, Lung, Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Abstract

PURPOSE
Mitofusin2 gene (Mfn2, also named Hyperplasia suppressive gene, HSG) is very important in the origin and development of hypertension. However, the mechanism of Mfn2/HSG expression regulation was not uncovered. This study was designed to explore the association of a novel 5'-uncoding region (UCR) -1248 A>G variation of HSG/Mfn2 gene and hypertension.
MATERIALS AND METHODS
472 healthy, normotensive subjects [normotension (NT) group], 454 prehypertensive subjects [prehypertension (PH) group] and 978 hypertensive patients [essential hypertension (EH) group] were screened for an association study between 5'-UCR -1248 A>G of Mfn2/HSG and hypertension by polymerase chain reaction and DNA sequencing after venous blood was drawn and DNA was extracted.
RESULTS
When comparing the A and G frequency in EH, PH and NT groups, in total, NT group significantly had higher A frequency than in PH group [odds ratio (OR)=1.605, confidence interval (CI) 95%=1.063-2.242, p=0.025] and EH group (OR=5.395, CI 95%=3.783-7.695, p<0.01). When subgrouped by gender, A frequency in NT group was still significantly higher than in EH group (male: OR=4.264, CI 95%=2.780-6.543, p<0.01; female: OR=8.897, CI 95%=4.686-16.891, p<0.01), but not from PH group, either in male group or in female group. Ordinal Logistic Regression analysis showed that A>G variation was significantly related with blood pressure level (B=-1.271, Wald=40.914, CI 95%=-1.660 - -0.881, p<0.01).
CONCLUSION
5'-UCR -1248 A>G variation of Mfn2/HSG gene was a novel variation and may be associated with hypertension in Chinese.

Keyword

Hyperplasia suppressor gene; Mitofusin-2; single nucleotide polymorphism; essential hypertension; transcription regulation

MeSH Terms

China
Female
GTP Phosphohydrolases/*genetics
Gene Expression Regulation
Genetic Association Studies
Genotype
Humans
Hypertension/*genetics
Logistic Models
Male
Mitochondrial Proteins/*genetics
*Polymorphism, Single Nucleotide
Sequence Analysis, DNA
Mitochondrial Proteins
GTP Phosphohydrolases

Figure

  • Fig. 1 The -1248 A>G variation of Mfn2 gene in 5'-uncoding region and the transcription factor binding site.


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