J Vet Sci.  2009 Dec;10(4):353-355. 10.4142/jvs.2009.10.4.353.

TP53 Polymorphisms allow for genetic sub-grouping of the canine transmissible venereal tumor

• Sanchez-Servin A
• Martinez S
• Cordova-Alarcon E
• Fajardo R

• KMID: 1726910
• DOI: http://doi.org/10.4142/jvs.2009.10.4.353

Abstract

The canine transmissible venereal tumor (CTVT) is found mainly in dogs' sexual organs. Currently, it is widely accepted that all samples of CTVT show similar histopathological characteristics and share common genetic alterations. Despite the common genetic origin of CTVT, mutations in the P53 gene have been reported. In this study, we proposed that tumor samples can be genetically grouped using this gene. The presence of different subgroups of CTVT was determined in Mexican dogs using the TP53 gene sequence in CTVT samples. Four new polymorphisms were found and therefore, the CTVT samples were classified in five subgroups.

Keyword

dog; polymorphisms; TP53; venereal tumors

MeSH Terms

Animals
Base Sequence
Dog Diseases/*genetics
Dogs
Gene Expression Regulation, Neoplastic/physiology
Molecular Sequence Data
Mutation
*Polymorphism, Genetic
Tumor Suppressor Protein p53/*genetics
Venereal Tumors, Veterinary/*genetics

Figure

• 

  Fig. 1 (A) Electropherograms of the sequence of the TP53 from canine transmissible venereal tumor (CTVT) samples, which show changes. Black arrows mark the change in nucleotides. (B) In the sequence alignment of the TP53 from CTVT samples, the nucleotide changes are marked in bold type. In the 5 samples of CTVT analyzed are observed four different mutations, producing three different alleles in the TP53 gene.

Reference

1. Calvert CA, Leifer CE, MacEwen EG. Vincristine for treatment of transmissible venereal tumor in the dog. J Am Vet Med Assoc. 1982. 181:163–164.

2. Choi YK, Kim CJ. Sequence analysis of canine LINE-1 elements and p53 gene in canine transmissible venereal tumor. J Vet Sci. 2002. 3:285–292.

3. Foster RA. McGavin MD, Zachary JF, editors. Female and male reproductive system. Pathologic Basis of Veterinary Disease. 2006. 4th ed. St. Louis: Elsevier;1263–1315.

4. Liao KW, Lin ZY, Pao HN, Kam SY, Wang FI, Chu RM. Identification of canine transmissible venereal tumor cells using in situ polymerase chain reaction and the stable sequence of the long interspersed nuclear element. J Vet Diagn Invest. 2003. 15:399–406.

5. Murgia C, Pritchard JK, Kim SY, Fassati A, Weiss RA. Clonal origin and evolution of a transmissible cancer. Cell. 2006. 126:477–487.

6. Oren M. Regulation of the p53 tumor suppressor protein. J Biol Chem. 1999. 274:36031–36034.

7. Oduye OO, Ikede BO, Esuruoso GO, Akpokodje JU. Metastatic transmissible venereal tumour in dogs. J Small Anim Pract. 1973. 14:625–637.

8. Rebbeck CA, Thomas R, Breen M, Leroi AM, Burt A. Origins and evolution of a transmissible cancer. Evolution. 2009. 63:2340–2349.

9. Setoguchi A, Sakai T, Okuda M, Minehata K, Yazawa M, Ishizaka T, Watari T, Nishimura R, Sasaki N, Hasegawa A, Tsujimoto H. Aberrations of the p53 tumor suppressor gene in various tumors in dogs. Am J Vet Res. 2001. 62:433–439.

10. Vázquez-Mota N, Simón-Martínez J, Córdova-Alarcon E, Lagunes L, Fajardo R. The T963C mutation of TP53 gene does not participate in the clonal origin of canine TVT. Vet Res Commun. 2008. 32:187–191.

11. Veldhoen N, Milner J. Isolation of canine p53 cDNA and detailed characterization of the full length canine p53 protein. Oncogene. 1998. 16:1077–1084.