Korean J Otorhinolaryngol-Head Neck Surg.  2014 Sep;57(9):603-609. 10.3342/kjorl-hns.2014.57.9.603.

The Effect of Omnaris(R) on the Inflammatory Reaction and Mucin Gene Expression from Nasal Polyp Epithelial Cells

Affiliations
  • 1Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Catholic University of Daegu, Daegu, Korea. hsseung@cu.ac.kr

Abstract

BACKGROUND AND OBJECTIVES
Glucocorticoids are a potent anti-inflammatory agent. The au-thors conducted this study to investigate the effect of Omnaris(R) on suppression of inflammation induction and mucin gene expression in nasal polyp epithelial cells.
SUBJECTS AND METHOD
Primary nasal polyp epithelial cells were stimulated by 5 ug/mL of streptococcal enterotoxin B (SEB) and lipopolysaccharide (LPS). To determine the effects of Omnaris(R), cells were pretreated with 200, 100, 10, 1 ng/mL of Omnaris(R). The anti-inflammatory effect of epithelial cells were confirmed by measuring interleukin (IL)-6, IL-8, and granulocyte-macrophage colony stimulating factor (GM-CSF), and mucin gene expressions were determined by real time PCR for MUC4, MUC5AC, MUC5B, MUC8.
RESULTS
SEB and LPS enhanced the production of IL-6, IL-8, and GM-CSF from nasal polyp epithelial cells. The increased cytokine levels were significantly suppressed by Omnaris(R) at 100 and 10 ng/mL. The expressions of MUC4, MUC5AC, MUC5B, MUC8 mRNA, and MUC4 mRNA were increased by SEB and LPS, respectively. The increased expression of these mucin genes were significantly suppressed by 100, 10, and 1 ng/mL of Omnaris(R).
CONCLUSION
Omnaris(R) significantly suppressed the production of chemical mediators and mucin gene expression, which indicated that Omnaris(R) is effective in improving and treating inflammatory diseases in the nasal cavity.

Keyword

Cytokine; Epithelial cell; Glucocorticoid; Mucin gene; Omnaris(R)

MeSH Terms

Colony-Stimulating Factors
Enterotoxins
Epithelial Cells*
Gene Expression*
Glucocorticoids
Granulocyte-Macrophage Colony-Stimulating Factor
Inflammation
Interleukin-6
Interleukin-8
Interleukins
Mucins*
Nasal Cavity
Nasal Polyps*
Real-Time Polymerase Chain Reaction
RNA, Messenger
Colony-Stimulating Factors
Enterotoxins
Glucocorticoids
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-6
Interleukin-8
Interleukins
Mucins
RNA, Messenger
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