Korean J Parasitol.  2004 Dec;42(4):169-174. 10.3347/kjp.2004.42.4.169.

Organ-specific antigens of Clonorchis sinensis

Affiliations
  • 1Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea. hst@snu.ac.kr
  • 2Department of Parasitology, College of Medicine, Yanbian University, 133000 Yanji, Jilin Province, P.R. China.

Abstract

This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7- and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26- and 28- kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43- and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

Keyword

Clonorchis sinensis; organ-specific antigen; immunoblot; 7-kDa antigen
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