Korean J Parasitol.
2011 Dec;49(4):431-436. 10.3347/kjp.2011.49.4.431.
Expression of Exogenous Human Hepatic Nuclear Factor-1alpha by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2
- Affiliations
-
- 1Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine, Iksan 570-749, Korea. hyunpk@wonkwang.ac.kr
- 2Department of Health Administration, Namseoul University, Cheonan 331-707, Korea.
- 3Department of Biomedical Laboratory Science, Molecular Diagnosis Research Institute, Namseoul University, Cheonan 331-707, Korea. syjung@nsu.ac.kr
- KMID: 1715752
- DOI: http://doi.org/10.3347/kjp.2011.49.4.431
Abstract
- The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice, liver injury follows parasite entry, persisting to the erythrocytic stage in the case of infection with the fatal strain of Plasmodium falciparum. Hepatic nuclear factor (HNF)-1alpha is a master regulator of not only the liver damage and adaptive responses but also diverse metabolic functions. In this study, we analyzed the expression of host HNF-1alpha in relation to malaria infection and evaluated its interaction with the 5'-untranslated region of subtilisin-like protease 2 (subtilase, Sub2). Recombinant human HNF-1alpha expressed by a lentiviral vector (LV HNF-1alpha) was introduced into mice. Interestingly, differences in the activity of the 5'-untranslated region of the Pf-Sub2 promoter were detected in 293T cells, and LV HNF-1alpha was observed to influence promoter activity, suggesting that host HNF-1alpha interacts with the Sub2 gene.
Keyword
MeSH Terms
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5' Untranslated Regions/*genetics
Animals
Cell Line
DNA, Protozoan/genetics
Gene Expression Regulation/*genetics
Genetic Vectors
Hepatocyte Nuclear Factor 1-alpha/administration & dosage/genetics/*metabolism
Host-Parasite Interactions
Humans
Injections, Intravenous
Lentivirus/genetics
Malaria, Falciparum/metabolism/*parasitology/pathology
Mice
Plasmodium falciparum/drug effects/*genetics
Promoter Regions, Genetic/genetics
RNA, Messenger/genetics
RNA, Protozoan/genetics
Recombinant Proteins
Signal Transduction
Subtilisins/*genetics/metabolism
5' Untranslated Regions
DNA, Protozoan
Hepatocyte Nuclear Factor 1-alpha
RNA, Messenger
RNA, Protozoan
Recombinant Proteins
Subtilisins
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