Korean J Parasitol.  2013 Aug;51(4):449-452. 10.3347/kjp.2013.51.4.449.

Genetic Variation of Taenia Pisiformis Collected from Sichuan, China, Based on the Mitochondrial Cytochrome b gene

• Yang D
• Ren Y
• Fu Y
• Xie Y
• Nie H
• Nong X
• Gu X
• Wang S
• Peng X
• Yang G

• KMID: 1715691
• DOI: http://doi.org/10.3347/kjp.2013.51.4.449

Abstract

Taenia pisiformis is one of the most important parasites of canines and rabbits. T. pisiformis cysticercus (the larval stage) causes severe damage to rabbit breeding, which results in huge economic losses. In this study, the genetic variation of T. pisiformis was determined in Sichuan Province, China. Fragments of the mitochondrial cytochrome b (cytb) (922 bp) gene were amplified in 53 isolates from 8 regions of T. pisiformis. Overall, 12 haplotypes were found in these 53 cytb sequences. Molecular genetic variations showed 98.4% genetic variation derived from intra-region. F(ST) and Nm values suggested that 53 isolates were not genetically differentiated and had low levels of genetic diversity. Neutrality indices of the cytb sequences showed the evolution of T. pisiformis followed a neutral mode. Phylogenetic analysis revealed no correlation between phylogeny and geographic distribution. These findings indicate that 53 isolates of T. pisiformis keep a low genetic variation, which provide useful knowledge for monitoring changes in parasite populations for future control strategies.

Keyword

Taenia pisiformis; cytochrome b; genetic variation; China

MeSH Terms

Animals
China
Cytochromes b/*genetics
*Genetic Variation
Helminth Proteins/*genetics
Humans
Mitochondria/*genetics
Molecular Sequence Data
Phylogeny
Rabbits
Taenia/classification/genetics/*isolation & purification
Taeniasis/*parasitology
Cytochromes b
Helminth Proteins