Korean J Parasitol.
2014 Feb;52(1):117-120. 10.3347/kjp.2014.52.1.117.
Partial Purification and Properties of a Cysteine Protease from Citrus Red Mite Panonychus citri
- Affiliations
-
- 1Department of Preventive Medicine and The Environmental Health Center, Jeju National University School of Medicine, Jeju 690-767, Korea.
- 2Department of Surgery, Jeju National University School of Medicine, Jeju 690-767, Korea.
- 3Department of Internal Medicine, Jeju National University School of Medicine, Jeju 690-767, Korea.
- 4Department of Internal Medicine, Gachon University Gil Medical Center, Incheon 406-799, Korea.
- 5Department of Parasitology, Jeju National University School of Medicine, Jeju 690-767, Korea. ybchung@jejunu.ac.kr
- KMID: 1715683
- DOI: http://doi.org/10.3347/kjp.2014.52.1.117
Abstract
- Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify and assess properties of a cysteine protease from the mites acting as a potentially pathogenic factor to citrus-cultivating farmers. A cysteine protease was purified using column chromatography of Mono Q anion exchanger and Superdex 200 HR gel filtration. It was estimated to be 46 kDa by gel filtration column chromatography and consisted of 2 polypeptides, at least. Cysteine protease inhibitors, such as trans poxy-succinyl-L-leucyl-amido (4-guanidino) butane (E-64) and iodoacetic acid (IAA) totally inhibited the enzyme activities, whereas serine or metalloprotease inhibitors did not affect the activities. In addition, the purified enzyme degraded human IgG, collagen, and fibronectin, but not egg albumin. From these results, the cysteine protease of the mites might be involved in the pathogenesis such as tissue destruction and penetration instead of nutrient digestion.
Keyword
MeSH Terms
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Animals
Chromatography, Gel
Chromatography, Ion Exchange
Collagen/metabolism
Cysteine Proteases/chemistry/*isolation & purification
Cysteine Proteinase Inhibitors/metabolism
Fibronectins/metabolism
Humans
Immunoglobulin G/metabolism
Molecular Weight
Protein Subunits/chemistry/isolation & purification
Proteolysis
Substrate Specificity
Tetranychidae/*enzymology
Collagen
Cysteine Proteases
Cysteine Proteinase Inhibitors
Fibronectins
Immunoglobulin G
Protein Subunits
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