Korean J Parasitol.  2011 Mar;49(1):79-83. 10.3347/kjp.2011.49.1.79.

Identification of Antigenic Proteins in Trichomonas vaginalis

Affiliations
  • 1Department of Environmental Medical Biology and Institute of Tropical Medicine, Post Brain Korea 21 Program, Yonsei University College of Medicine, Seoul 120-752, Korea. sjpark615@yuhs.ac
  • 2Department of Environmental Medical Biology, Hanyang University College of Medicine, Seoul 133-791, Korea.

Abstract

Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T. vaginalis surface proteins were investigated using polyclonal antibodies specific to the membrane fractions of T. vaginalis. The T. vaginalis expression library was constructed by cloning the cDNA derived from mRNA of T. vaginalis into a phage lambda Uni-ZAP XR vector, and then used for immunoscreening with the anti-membrane proteins of T. vaginalis antibodies. The immunoreactive proteins identified included adhesion protein AP65-1, alpha-actinin, kinesin-associated protein, teneurin, and 2 independent hypothetical proteins. Immunofluorescence assays showed that AP65-1, one of the identified immunogenic clones, is prevalent in the whole body of T. vaginalis. This study led us to identify T. vaginalis proteins which may stimulate immune responses by human cells.

Keyword

Trichomonas vaginalis; antigenic protein; membrane protein; immunoscreening
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