Korean J Parasitol.  1996 Dec;34(4):215-224. 10.3347/kjp.1996.34.4.215.

Localization of cytoskeletal proteins in Cryptosporidium parvum using double immunogold labeling

Affiliations
  • 1Department of Parasitology, College of Medicine, Kon-Kuk University, Choongju 380-701,Korea
  • 2Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea

Abstract

Actin and some actin binding proteins such as tropomyosin, -actinin and troponin T were localized by simultaneous double immunogold labeling in several developmental stages of Cryptosporidium parvum. All of the observed developmental stages have many particles of tropomyosin and actin around pellicle and cytoplasm. Tropomyosin was labeled much more than the actin when these two proteins were labeled simultaneously. And alpha-actinin was labeled mostly in the pellicle, but troponin T labeling was very rarely observed. From this study, it was suggested that tropomyosin seems to be one of the major proteins of C. parvum, so it must be playing important roles in C. parvum.

Keyword

actin; tropomyosin; alpha-actinin; troponin T

MeSH Terms

parasitology-protozoa
actin
tropomyosin
alpha-actinin
troponin T
Cryptosporidium parvum
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