Gut Liver.
2013 Nov;7(6):688-695.
An Inverse Relationship between the Expression of the Gastric Tumor Suppressor RUNX3 and Infection with Helicobacter pylori in Gastric Epithelial Dysplasia
- Affiliations
-
- 1Department of Internal Medicine, St. Vincent's Hospital, The Catholic University of Korea College of Medicine, Suwon, Korea.
- 2Department of Internal Medicine, St. Paul's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea. shjung74@catholic.ac.kr
- 3Department of Pathology, St. Vincent's Hospital, The Catholic University of Korea College of Medicine, Suwon, Korea.
- 4Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Abstract
- BACKGROUND/AIMS
This study was performed to determine the association between RUNX3 expression and Helicobacter pylori infection in premalignant gastric lesions.
METHODS
We examined 107 patients with gastric epithelial dysplasia who had undergone endoscopic mucosal resection or submucosal dissection. All tissue samples were evaluated by RUNX3 staining and subclassified by immunophenotype. H. pylori infection in dysplastic lesions and the normal surrounding tissue was examined by silver staining, and cagA status was assessed by polymerase chain reaction.
RESULTS
The loss of RUNX3 expression was observed in 62 cases (57.9%), and an association with H. pylori infection was found in 54 cases (50.5%). The infection rate with the cagA-positive H. pylori strain was 63.0%. In RUNX3-negative lesions, the rate of H. pylori infection (p=0.03) and the frequency of category 4 lesions (according to the revised Vienna classification) were high (p=0.02). In addition, the gastric mucin phenotype was predominant. In RUNX3-negative category 4 lesions, the rate of cagA-positive H. pylori infection rate was high but not significantly increased (p=0.08).
CONCLUSIONS
Infection with H. pylori is associated with inactivation of RUNX3 in early gastric carcinogenesis. This mechanism was prominent in gastric cancer with a gastric mucin phenotype.
MeSH Terms
-
Adenoma/*chemistry
Aged
Antigens, Bacterial/genetics
Bacterial Proteins/genetics
Carcinoma/*chemistry
Cell Transformation, Neoplastic
Core Binding Factor Alpha 3 Subunit/*analysis
Female
Gastric Mucosa/*chemistry/pathology
Helicobacter Infections/*metabolism
Helicobacter pylori/*genetics
Humans
Male
Middle Aged
Mucin 5AC/analysis
Mucin-2/analysis
Mucin-6/analysis
Neprilysin/analysis
Phenotype
Precancerous Conditions/*chemistry/pathology
Stomach Neoplasms/*chemistry
Antigens, Bacterial
Bacterial Proteins
Core Binding Factor Alpha 3 Subunit
Mucin 5AC
Mucin-2
Mucin-6
Neprilysin
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