Korean J Parasitol.  2013 Dec;51(6):743-745. 10.3347/kjp.2013.51.6.743.

Modified Formalin-Ether Concentration Technique for Diagnosis of Human Strongyloidiasis

Affiliations
  • 1Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand. awitthay@wu.ac.th
  • 2Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

Abstract

We compared the efficacy and applicability of a modified formalin-ether concentration technique (M-FECT) to the conventional FECT (C-FECT) and the agar plate culture (APC) method for the detection of Strongyloides stercoralis larvae. For this purpose
, we used 600 human fecal specimens collected in an endemic area of southern Thailand. In the M-FECT, we used 2 layers of wire meshes, instead of gauze, to avoid the loss by absorption/adhesion of larvae to the gauze during filtration, and we reduced the exposure time of S. stercoralis larvae in stool samples to formalin. By such simple modifications, the efficacy of M-FECT has become comparable to APC and was much better than that of C-FECT for the diagnosis of strongyloidiasis.

Keyword

Strongyloides stercoralis; formalin-ether concentration technique; agar plate culture; feces; diagnosis
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