Korean J Parasitol.  2013 Dec;51(6):719-726. 10.3347/kjp.2013.51.6.719.

Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes

Affiliations
  • 1Department of Parasitology, Medical Research Institute and Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju 361-763, Korea. kseom@chungbuk.ac.kr
  • 2Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
  • 3Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-70-51, Korea.
  • 4Department of Environmental Medical Biology, Institute of Tropical Medicine and Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul 120-752, Korea.
  • 5Department of Immunology and Microbiology, Eulji University School of Medicine, Daejeon 301-746, Korea.
  • 6Department of Parasitology, Korea University College of Medicine, Seoul 136-705, Korea.

Abstract

Mitochondrial genomes have been extensively studied for phylogenetic purpose
s and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.

Keyword

Haplorchis taichui; trematode; mitochondrial genome; molecular phylogeny
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